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Human PYY ELISA Kit

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  • Alternative name

    Human PYY ELISA Kit;Human PYY-I ELISA Kit;Human peptide tyrosine tyrosine ELISA Kit;Human PYY-II ELISA Kit;Human PYY1 ELISA Kit;Human peptide YY ELISA Kit;Human prepro-PYY ELISA Kit;
  • Catalog
    E028547
  • species
    Human
  • GenePYY
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman PYY ELISA Kit allows for the in vitro quantitative determination of PYY , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical    Principle of the Assay: PYY ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PYY antibody and an PYY-HRP conjugate. The assay sample and buffer are incubated together with PYY-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PYY concentration since PYY from samples and PYY-HRP conjugate compete for the anti-PYY antibody binding site. Since the number of sites is limited, as more sites are occupied by PYY from the sample, fewer sites are left to bind PYY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PYY concentration in each sample is interpolated from this standard curve.
  • Human Peptide YY(3-36) Protein information
  • Uniprot ID PYY_HUMAN
  • Uniprot AC P10082; Q5U5Q6; Q6FGH8;
  • UniGene Hs.169249;
  • GeneID 5697
  • KEGG hsa:5697;
  • Human Peptide YY(3-36) Protein SEQUENCE

  • SEQUENCE 97 AA; 11145 MW; C166B73412E356B1 CRC64;

    MVFVRRPWPA LTTVLLALLV CLGALVDAYP IKPEAPREDA SPEELNRYYA

    SLRHYLNLVT RQRYGKRDGP DTLLSKTFFP DGEDRPVRSR SEGPDLW

  • UCSC uc002ieq.4; human. [P10082-1];



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