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Human PADI4 ELISA Kit

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  • Alternative name

    Human HL-60 PAD ELISA Kit;Human Peptidylarginine deiminase IV ELISA Kit;Human protein-arginine deiminase type IV ELISA Kit;Human PAD4 ELISA Kit;Human PADI5 ELISA Kit;Human PDI5 ELISA Kit;Human PAD ELISA Kit;Human PDI4 ELISA Kit;Human peptidyl arginine deiminase 4 ELISA Kit;Human protein-arginine deiminase type-4 ELISA Kit;Human PADI-H protein ELISA Kit;Human peptidyl arginine deiminase, type IV ELISA Kit;Human peptidyl arginine deiminase, type V ELISA Kit;
  • Catalog
    E028569
  • species
    Human
  • GenePADI4
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Peptidyl Arginine Deiminase Type IV (PADI4). No significant cross-reactivity or interference between Peptidyl Arginine Deiminase Type IV (PADI4) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.121ng/mL.
  • Intended UseHuman PADI4 ELISA Kit allows for the in vitro quantitative determination of PADI4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidyl Arginine Deiminase Type IV (PADI4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidyl Arginine Deiminase Type IV (PADI4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidyl Arginine Deiminase Type IV (PADI4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peptidyl Arginine Deiminase Type IV (PADI4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein-arginine deiminase type-4 Protein information
  • Uniprot ID PADI4_HUMAN
  • Uniprot AC Q9UM07; A8K392; B2RBW0; Q5VTZ8; Q70SX4;
  • UniGene Hs.522969;
  • GeneID 23569
  • KEGG hsa:23569;
  • Human Protein-arginine deiminase type-4 Protein SEQUENCE

  • SEQUENCE 663 AA; 74079 MW; 79D7AF7B3D307A3B CRC64;

    MAQGTLIRVT PEQPTHAVCV LGTLTQLDIC SSAPEDCTSF SINASPGVVV

    DIAHGPPAKK KSTGSSTWPL DPGVEVTLTM KVASGSTGDQ KVQISYYGPK

    TPPVKALLYL TGVEISLCAD ITRTGKVKPT RAVKDQRTWT WGPCGQGAIL

    LVNCDRDNLE SSAMDCEDDE VLDSEDLQDM SLMTLSTKTP KDFFTNHTLV

    LHVARSEMDK VRVFQATRGK LSSKCSVVLG PKWPSHYLMV PGGKHNMDFY

    VEALAFPDTD FPGLITLTIS LLDTSNLELP EAVVFQDSVV FRVAPWIMTP

    NTQPPQEVYA CSIFENEDFL KSVTTLAMKA KCKLTICPEE ENMDDQWMQD

    EMEIGYIQAP HKTLPVVFDS PRNRGLKEFP IKRVMGPDFG YVTRGPQTGG

    ISGLDSFGNL EVSPPVTVRG KEYPLGRILF GDSCYPSNDS RQMHQALQDF

    LSAQQVQAPV KLYSDWLSVG HVDEFLSFVP APDRKGFRLL LASPRSCYKL

    FQEQQNEGHG EALLFEGIKK KKQQKIKNIL SNKTLREHNS FVERCIDWNR

    ELLKRELGLA ESDIIDIPQL FKLKEFSKAE AFFPNMVNML VLGKHLGIPK

    PFGPVINGRC CLEEKVCSLL EPLGLQCTFI NDFFTYHIRH GEVHCGTNVR

    RKPFSFKWWN MVP

  • UCSC uc001baj.3; human.;



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