Human PPARD ELISA Kit

Alternative name
Human PPAR-delta ELISA Kit;Human NUCI ELISA Kit;Human nuclear hormone receptor 1 ELISA Kit;Human NUC1 ELISA Kit;Human nuclear receptor subfamily 1 group C member 2 ELISA Kit;Human peroxisome proliferator-activated receptor beta ELISA Kit;Human PPAR-beta ELISA Kit;Human NR1C2 ELISA Kit;Human PPARB ELISA Kit;Human FAAR ELISA Kit;Human NUCII ELISA Kit;Human peroxisome proliferator activated receptor delta ELISA Kit;Human peroxisome proliferator-activated receptor delta ELISA Kit;Human peroxisome proliferator-activated nuclear receptor beta/delta variant 2 ELISA Kit;
Catalog
E028745
species
Human
GenePPARD
SpecificityThis assay has high sensitivity and excellent specificity for detection of PPARd. No significant cross-reactivity or interference between PPARd and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human PPARd is typically less than 0.057ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by add
Intended UseHuman PPARD ELISA Kit allows for the in vitro quantitative determination of PPARD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPARd. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PPARd. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PPARd, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PPARd in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Peroxisome proliferator-activated receptor delta Protein information
Uniprot ID PPARD_HUMAN
Uniprot AC Q9BUD4;
UniGene Hs.696032;
GeneID 5467
KEGG hsa:5467;
Human Peroxisome proliferator-activated receptor delta Protein SEQUENCE
SEQUENCE 441 AA; 49903 MW; 94FBB2A4B46521E8 CRC64;
MEQPQEEAPE VREEEEKEEV AEAEGAPELN GGPQHALPSS SYTDLSRSSS
PPSLLDQLQM GCDGASCGSL NMECRVCGDK ASGFHYGVHA CEGCKGFFRR
TIRMKLEYEK CERSCKIQKK NRNKCQYCRF QKCLALGMSH NAIRFGRMPE
AEKRKLVAGL TANEGSQYNP QVADLKAFSK HIYNAYLKNF NMTKKKARSI
LTGKASHTAP FVIHDIETLW QAEKGLVWKQ LVNGLPPYKE ISVHVFYRCQ
CTTVETVREL TEFAKSIPSF SSLFLNDQVT LLKYGVHEAI FAMLASIVNK
DGLLVANGSG FVTREFLRSL RKPFSDIIEP KFEFAVKFNA LELDDSDLAL
FIAAIILCGD RPGLMNVPRV EAIQDTILRA LEFHLQANHP DAQYLFPKLL
QKMADLRQLV TEHAQMMQRI KKTETETSLH PLLQEIYKDM Y
UCSC uc003okm.3; human. [Q03181-1];

Category

Human PPARD ELISA Kit