Human PPARGC1A ELISA Kit

Alternative name
Human PGC-1-alpha ELISA Kit;Human PPAR-gamma coactivator 1-alpha ELISA Kit;Human PPARGC-1-alpha ELISA Kit;Human Ligand effect modulator 6 ELISA Kit;Human LEM6 ELISA Kit;Human PGC1 ELISA Kit;Human PGC1A ELISA Kit;Human PPARGC1 ELISA Kit;Human PGC-1(alpha) ELISA Kit;Human PGC-1alpha ELISA Kit;Human PGC-1v ELISA Kit;Human PPARG coactivator 1 alpha ELISA Kit;Human peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit;Human L-PGC-1alpha ELISA Kit;Human PPAR gamma coactivator variant form ELISA Kit;Human PPARgamma coactivator 1alpha ELISA Kit;Human ligand effect modulator-6 ELISA Kit;Human peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext ELISA Kit;Human peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a ELISA Kit;Human peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT ELISA Kit;
Catalog
E028756
species
Human
GenePPARGC1A
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PPARGC1A. No significant cross-reactivity or interference between Human PPARGC1A and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the crossreactivity detection between Human PPARGC1A and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman PPARGC1A ELISA Kit allows for the in vitro quantitative determination of PPARGC1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PPARGC1A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PPARGC1A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PPARGC1A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PPARGC1A can be calculated.
Human Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Protein information
Uniprot ID PRGC1_HUMAN
Uniprot AC Q3LIG1; Q4W5M7; Q9UN32;
UniGene Hs.527078;
GeneID 10891
KEGG hsa:10891;
Human Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Protein SEQUENCE
SEQUENCE 798 AA; 91027 MW; 97DDD3643051A5F1 CRC64;
MAWDMCNQDS ESVWSDIECA ALVGEDQPLC PDLPELDLSE LDVNDLDTDS
FLGGLKWCSD QSEIISNQYN NEPSNIFEKI DEENEANLLA VLTETLDSLP
VDEDGLPSFD ALTDGDVTTD NEASPSSMPD GTPPPQEAEE PSLLKKLLLA
PANTQLSYNE CSGLSTQNHA NHNHRIRTNP AIVKTENSWS NKAKSICQQQ
KPQRRPCSEL LKYLTTNDDP PHTKPTENRN SSRDKCTSKK KSHTQSQSQH
LQAKPTTLSL PLTPESPNDP KGSPFENKTI ERTLSVELSG TAGLTPPTTP
PHKANQDNPF RASPKLKSSC KTVVPPPSKK PRYSESSGTQ GNNSTKKGPE
QSELYAQLSK SSVLTGGHEE RKTKRPSLRL FGDHDYCQSI NSKTEILINI
SQELQDSRQL ENKDVSSDWQ GQICSSTDSD QCYLRETLEA SKQVSPCSTR
KQLQDQEIRA ELNKHFGHPS QAVFDDEADK TGELRDSDFS NEQFSKLPMF
INSGLAMDGL FDDSEDESDK LSYPWDGTQS YSLFNVSPSC SSFNSPCRDS
VSPPKSLFSQ RPQRMRSRSR SFSRHRSCSR SPYSRSRSRS PGSRSSSRSC
YYYESSHYRH RTHRNSPLYV RSRSRSPYSR RPRYDSYEEY QHERLKREEY
RREYEKRESE RAKQRERQRQ KAIEERRVIY VGKIRPDTTR TELRDRFEVF
GEIEECTVNL RDDGDSYGFI TYRYTCDAFA ALENGYTLRR SNETDFELYF
CGRKQFFKSN YADLDSNSDD FDPASTKSKY DSLDFDSLLK EAQRSLRR
UCSC uc003gqs.3; human. [Q9UBK2-1];

Category

Human PPARGC1A ELISA Kit