Human PNMT ELISA Kit

Alternative name
Human PNMTase ELISA Kit;Human noradrenaline N-methyltransferase ELISA Kit;Human PENT ELISA Kit;Human phenylethanolamine N-methyltransferase ELISA Kit;Human phenylethanolamine N-methylase ELISA Kit;
Catalog
E028854
species
Human
GenePNMT
SpecificityThis assay has high sensitivity and excellent specificity for detection of human PNMT. No significant cross-reactivity or interference between human PNMT and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human PNMT is typically less than 9.75 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseHuman PNMT ELISA Kit allows for the in vitro quantitative determination of PNMT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PNMT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PNMT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PNMT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNMT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Phenylethanolamine N-methyltransferase Protein information
Uniprot ID PNMT_HUMAN
Uniprot AC P11086;
UniGene Hs.1892;
GeneID 5409
KEGG hsa:5409;
Human Phenylethanolamine N-methyltransferase Protein SEQUENCE
SEQUENCE 282 AA; 30855 MW; 56F3A9981D9ABF4C CRC64;
MSGADRSPNA GAAPDSAPGQ AAVASAYQRF EPRAYLRNNY APPRGDLCNP
NGVGPWKLRC LAQTFATGEV SGRTLIDIGS GPTVYQLLSA CSHFEDITMT
DFLEVNRQEL GRWLQEEPGA FNWSMYSQHA CLIEGKGECW QDKERQLRAR
VKRVLPIDVH QPQPLGAGSP APLPADALVS AFCLEAVSPD LASFQRALDH
ITTLLRPGGH LLLIGALEES WYLAGEARLT VVPVSEEEVR EALVRSGYKV
RDLRTYIMPA HLQTGVDDVK GVFFAWAQKV GL
UCSC uc002hsi.3; human.;

Category

Human PNMT ELISA Kit

Human PNMTase ELISA Kit;Human noradrenaline N-methyltransferase ELISA Kit;Human PENT ELISA Kit;Human phenylethanolamine N-methyltransferase ELISA Kit;Human phenylethanolamine N-methylase ELISA Kit;