Human PICALM ELISA Kit

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  • Alternative name

    Human clathrin assembly lymphoid myeloid leukemia protein ELISA Kit;Human CALM ELISA Kit;Human CLTH ELISA Kit;Human LAP ELISA Kit;Human phosphatidylinositol binding clathrin assembly protein ELISA Kit;Human phosphatidylinositol-binding clathrin assembly protein ELISA Kit;

  • Catalog
    E028938
  • species
    Human
  • GenePICALM
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PICALM. No significant cross-reactivity or interference between PICALM and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human PICALM is typically less than 0.126ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by ad
  • Intended UseHuman PICALM ELISA Kit allows for the in vitro quantitative determination of PICALM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PICALM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PICALM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PICALM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PICALM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Phosphatidylinositol-binding clathrin assembly protein Protein information
  • Uniprot ID PICAL_HUMAN
  • Uniprot AC Q13492; B4DTM3; E9PN05; F8VPG7; O60700; Q4LE54; Q6GMQ6; Q86XZ9;
  • UniGene Hs.163893;
  • GeneID 8301
  • KEGG hsa:8301;
  • Human Phosphatidylinositol-binding clathrin assembly protein Protein SEQUENCE
  • SEQUENCE 652 AA; 70755 MW; AC3227E9D32AFEDA CRC64;

    MSGQSLTDRI TAAQHSVTGS AVSKTVCKAT THEIMGPKKK HLDYLIQCTN

    EMNVNIPQLA DSLFERTTNS SWVVVFKSLI TTHHLMVYGN ERFIQYLASR

    NTLFNLSNFL DKSGLQGYDM STFIRRYSRY LNEKAVSYRQ VAFDFTKVKR

    GADGVMRTMN TEKLLKTVPI IQNQMDALLD FNVNSNELTN GVINAAFMLL

    FKDAIRLFAA YNEGIINLLE KYFDMKKNQC KEGLDIYKKF LTRMTRISEF

    LKVAEQVGID RGDIPDLSQA PSSLLDALEQ HLASLEGKKI KDSTAASRAT

    TLSNAVSSLA STGLSLTKVD EREKQAALEE EQARLKALKE QRLKELAKKP

    HTSLTTAASP VSTSAGGIMT APAIDIFSTP SSSNSTSKLP NDLLDLQQPT

    FHPSVHPMST ASQVASTWGD PFSATVDAVD DAIPSLNPFL TKSSGDVHLS

    ISSDVSTFTT RTPTHEMFVG FTPSPVAQPH PSAGLNVDFE SVFGNKSTNV

    IVDSGGFDEL GGLLKPTVAS QNQNLPVAKL PPSKLVSDDL DSSLANLVGN

    LGIGNGTTKN DVNWSQPGEK KLTGGSNWQP KVAPTTAWNA ATMAPPVMAY

    PATTPTGMIG YGIPPQMGSV PVMTQPTLIY SQPVMRPPNP FGPVSGAQIQ

    FM

  • UCSC uc001pbl.4; human. [Q13492-1];



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