Human PIK3CA ELISA Kit

Alternative name
Human PI3-kinase subunit alpha ELISA Kit;Human PI3K-alpha ELISA Kit;Human PI3Kalpha ELISA Kit;Human PtdIns-3-kinase subunit alpha ELISA Kit;Human Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha ELISA Kit;Human ptdIns-3-kinase subunit p110-alpha ELISA Kit;Human p110alpha ELISA Kit;Human Phosphoinositide-3-kinase catalytic alpha polypeptide ELISA Kit;Human serine/threonine protein kinase PIK3CA ELISA Kit;Human CLOVE ELISA Kit;Human CWS5 ELISA Kit;Human MCAP ELISA Kit;Human MCM ELISA Kit;Human MCMTC ELISA Kit;Human PI3K ELISA Kit;Human p110-alpha ELISA Kit;Human phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha ELISA Kit;Human phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform ELISA Kit;Human PI3-kinase p110 subunit alpha ELISA Kit;Human phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha ELISA Kit;Human phosphatidylinositol 3-kinase, catalytic, alpha polypeptide ELISA Kit;Human phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha ELISA Kit;Human phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform ELISA Kit;Human phosphoinositide-3-kinase, catalytic, alpha polypeptide ELISA Kit;
Catalog
E028964
species
Human
GenePIK3CA
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman PIK3CA ELISA Kit allows for the in vitro quantitative determination of PIK3CA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PIK3CAantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PIK3CA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PIK3CA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman PIK3CAcan be calculated.
Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Protein information
Uniprot ID PK3CA_HUMAN
Uniprot AC P42336; Q14CW1; Q99762;
UniGene Hs.553498; Hs.715194; Hs.732394;
GeneID 5290
KEGG hsa:5290;
Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Protein SEQUENCE
SEQUENCE 1068 AA; 124284 MW; 041487231A9A1207 CRC64;
MPPRPSSGEL WGIHLMPPRI LVECLLPNGM IVTLECLREA TLITIKHELF
KEARKYPLHQ LLQDESSYIF VSVTQEAERE EFFDETRRLC DLRLFQPFLK
VIEPVGNREE KILNREIGFA IGMPVCEFDM VKDPEVQDFR RNILNVCKEA
VDLRDLNSPH SRAMYVYPPN VESSPELPKH IYNKLDKGQI IVVIWVIVSP
NNDKQKYTLK INHDCVPEQV IAEAIRKKTR SMLLSSEQLK LCVLEYQGKY
ILKVCGCDEY FLEKYPLSQY KYIRSCIMLG RMPNLMLMAK ESLYSQLPMD
CFTMPSYSRR ISTATPYMNG ETSTKSLWVI NSALRIKILC ATYVNVNIRD
IDKIYVRTGI YHGGEPLCDN VNTQRVPCSN PRWNEWLNYD IYIPDLPRAA
RLCLSICSVK GRKGAKEEHC PLAWGNINLF DYTDTLVSGK MALNLWPVPH
GLEDLLNPIG VTGSNPNKET PCLELEFDWF SSVVKFPDMS VIEEHANWSV
SREAGFSYSH AGLSNRLARD NELRENDKEQ LKAISTRDPL SEITEQEKDF
LWSHRHYCVT IPEILPKLLL SVKWNSRDEV AQMYCLVKDW PPIKPEQAME
LLDCNYPDPM VRGFAVRCLE KYLTDDKLSQ YLIQLVQVLK YEQYLDNLLV
RFLLKKALTN QRIGHFFFWH LKSEMHNKTV SQRFGLLLES YCRACGMYLK
HLNRQVEAME KLINLTDILK QEKKDETQKV QMKFLVEQMR RPDFMDALQG
FLSPLNPAHQ LGNLRLEECR IMSSAKRPLW LNWENPDIMS ELLFQNNEII
FKNGDDLRQD MLTLQIIRIM ENIWQNQGLD LRMLPYGCLS IGDCVGLIEV
VRNSHTIMQI QCKGGLKGAL QFNSHTLHQW LKDKNKGEIY DAAIDLFTRS
CAGYCVATFI LGIGDRHNSN IMVKDDGQLF HIDFGHFLDH KKKKFGYKRE
RVPFVLTQDF LIVISKGAQE CTKTREFERF QEMCYKAYLA IRQHANLFIN
LFSMMLGSGM PELQSFDDIA YIRKTLALDK TEQEALEYFM KQMNDAHHGG
WTTKMDWIFH TIKQHALN
UCSC uc003fjk.4; human.;

Category

Human PIK3CA ELISA Kit