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Human PIK3CD ELISA Kit

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  • Alternative name

    Human PI3-kinase subunit delta ELISA Kit;Human PI3K-delta ELISA Kit;Human PI3Kdelta ELISA Kit;Human PtdIns-3-kinase subunit delta ELISA Kit;Human Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta ELISA Kit;Human ptdIns-3-kinase subunit p110-delta ELISA Kit;Human P110DELTA ELISA Kit;Human APDS ELISA Kit;Human IMD14 ELISA Kit;Human PI3K ELISA Kit;Human p110D ELISA Kit;Human phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta ELISA Kit;Human phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform ELISA Kit;Human PI3-kinase p110 subunit delta ELISA Kit;Human phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta ELISA Kit;Human phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform ELISA Kit;Human phosphoinositide-3-kinase C ELISA Kit;Human phosphoinositide-3-kinase, catalytic, delta polypeptide variant p37delta ELISA Kit;
  • Catalog
    E029121
  • species
    Human
  • GenePIK3CD
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd). No significant cross-reactivity or interference between Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.112ng/mL.
  • Intended UseHuman PIK3CD ELISA Kit allows for the in vitro quantitative determination of PIK3CD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform Protein information
  • Uniprot ID PK3CD_HUMAN
  • Uniprot AC O00329; A6NCG0; G1FFP1; O15445; Q5SR49;
  • UniGene Hs.518451;
  • GeneID 5293
  • KEGG hsa:5293;
  • Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform Protein SEQUENCE

  • SEQUENCE 1044 AA; 119479 MW; A38B5D1A1081A3D0 CRC64;

    MPPGVDCPME FWTKEENQSV VVDFLLPTGV YLNFPVSRNA NLSTIKQLLW

    HRAQYEPLFH MLSGPEAYVF TCINQTAEQQ ELEDEQRRLC DVQPFLPVLR

    LVAREGDRVK KLINSQISLL IGKGLHEFDS LCDPEVNDFR AKMCQFCEEA

    AARRQQLGWE AWLQYSFPLQ LEPSAQTWGP GTLRLPNRAL LVNVKFEGSE

    ESFTFQVSTK DVPLALMACA LRKKATVFRQ PLVEQPEDYT LQVNGRHEYL

    YGSYPLCQFQ YICSCLHSGL TPHLTMVHSS SILAMRDEQS NPAPQVQKPR

    AKPPPIPAKK PSSVSLWSLE QPFRIELIQG SKVNADERMK LVVQAGLFHG

    NEMLCKTVSS SEVSVCSEPV WKQRLEFDIN ICDLPRMARL CFALYAVIEK

    AKKARSTKKK SKKADCPIAW ANLMLFDYKD QLKTGERCLY MWPSVPDEKG

    ELLNPTGTVR SNPNTDSAAA LLICLPEVAP HPVYYPALEK ILELGRHSEC

    VHVTEEEQLQ LREILERRGS GELYEHEKDL VWKLRHEVQE HFPEALARLL

    LVTKWNKHED VAQMLYLLCS WPELPVLSAL ELLDFSFPDC HVGSFAIKSL

    RKLTDDELFQ YLLQLVQVLK YESYLDCELT KFLLDRALAN RKIGHFLFWH

    LRSEMHVPSV ALRFGLILEA YCRGSTHHMK VLMKQGEALS KLKALNDFVK

    LSSQKTPKPQ TKELMHLCMR QEAYLEALSH LQSPLDPSTL LAEVCVEQCT

    FMDSKMKPLW IMYSNEEAGS GGSVGIIFKN GDDLRQDMLT LQMIQLMDVL

    WKQEGLDLRM TPYGCLPTGD RTGLIEVVLR SDTIANIQLN KSNMAATAAF

    NKDALLNWLK SKNPGEALDR AIEEFTLSCA GYCVATYVLG IGDRHSDNIM

    IRESGQLFHI DFGHFLGNFK TKFGINRERV PFILTYDFVH VIQQGKTNNS

    EKFERFRGYC ERAYTILRRH GLLFLHLFAL MRAAGLPELS CSKDIQYLKD

    SLALGKTEEE ALKHFRVKFN EALRESWKTK VNWLAHNVSK DNRQ

  • UCSC uc001aqb.5; human. [O00329-1];



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