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Human PIK3C3 ELISA Kit

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  • Alternative name

    Human PI3-kinase type 3 ELISA Kit;Human PI3K type 3 ELISA Kit;Human PtdIns-3-kinase type 3 ELISA Kit;Human phosphatidylinositol 3-kinase p100 subunit ELISA Kit;Human Phosphoinositide-3-kinase class 3 ELISA Kit;Human hVps34 ELISA Kit;Human Vps34 ELISA Kit;Human phosphatidylinositol 3-kinase catalytic subunit type 3 ELISA Kit;Human phosphoinositide-3-kinase, class 3 ELISA Kit;
  • Catalog
    E029122
  • species
    Human
  • GenePIK3C3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Phosphoinositide-3-Kinase Class 3 (PIK3C3). No significant cross-reactivity or interference between Phosphoinositide-3-Kinase Class 3 (PIK3C3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.59ng/mL.
  • Intended UseHuman PIK3C3 ELISA Kit allows for the in vitro quantitative determination of PIK3C3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoinositide-3-Kinase Class 3 (PIK3C3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoinositide-3-Kinase Class 3 (PIK3C3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoinositide-3-Kinase Class 3 (PIK3C3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoinositide-3-Kinase Class 3 (PIK3C3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Phosphatidylinositol 3-kinase catalytic subunit type 3 Protein information
  • Uniprot ID PK3C3_HUMAN
  • Uniprot AC Q8NEB9; Q15134;
  • UniGene Hs.464971; Hs.656958;
  • GeneID 5289
  • KEGG hsa:5289;
  • Human Phosphatidylinositol 3-kinase catalytic subunit type 3 Protein SEQUENCE

  • SEQUENCE 887 AA; 101549 MW; 1C03D97338E44976 CRC64;

    MGEAEKFHYI YSCDLDINVQ LKIGSLEGKR EQKSYKAVLE DPMLKFSGLY

    QETCSDLYVT CQVFAEGKPL ALPVRTSYKA FSTRWNWNEW LKLPVKYPDL

    PRNAQVALTI WDVYGPGKAV PVGGTTVSLF GKYGMFRQGM HDLKVWPNVE

    ADGSEPTKTP GRTSSTLSED QMSRLAKLTK AHRQGHMVKV DWLDRLTFRE

    IEMINESEKR SSNFMYLMVE FRCVKCDDKE YGIVYYEKDG DESSPILTSF

    ELVKVPDPQM SMENLVESKH HKLARSLRSG PSDHDLKPNA ATRDQLNIIV

    SYPPTKQLTY EEQDLVWKFR YYLTNQEKAL TKFLKCVNWD LPQEAKQALE

    LLGKWKPMDV EDSLELLSSH YTNPTVRRYA VARLRQADDE DLLMYLLQLV

    QALKYENFDD IKNGLEPTKK DSQSSVSENV SNSGINSAEI DSSQIITSPL

    PSVSSPPPAS KTKEVPDGEN LEQDLCTFLI SRACKNSTLA NYLYWYVIVE

    CEDQDTQQRD PKTHEMYLNV MRRFSQALLK GDKSVRVMRS LLAAQQTFVD

    RLVHLMKAVQ RESGNRKKKN ERLQALLGDN EKMNLSDVEL IPLPLEPQVK

    IRGIIPETAT LFKSALMPAQ LFFKTEDGGK YPVIFKHGDD LRQDQLILQI

    ISLMDKLLRK ENLDLKLTPY KVLATSTKHG FMQFIQSVPV AEVLDTEGSI

    QNFFRKYAPS ENGPNGISAE VMDTYVKSCA GYCVITYILG VGDRHLDNLL

    LTKTGKLFHI DFGYILGRDP KPLPPPMKLN KEMVEGMGGT QSEQYQEFRK

    QCYTAFLHLR RYSNLILNLF SLMVDANIPD IALEPDKTVK KVQDKFRLDL

    SDEEAVHYMQ SLIDESVHAL FAAVVEQIHK FAQYWRK

  • UCSC uc002lap.4; human.;



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