Human PLD2 ELISA Kit

Alternative name
Human PLD 2 ELISA Kit;Human hPLD2 ELISA Kit;Human choline phosphatase 2 ELISA Kit;Human PLD1C ELISA Kit;Human phosphatidylcholine-hydrolyzing phospholipase D2 ELISA Kit;Human phospholipase D2 ELISA Kit;
Catalog
E029238
species
Human
GenePLD2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Phospholipase D2 (PLD2). No significant cross-reactivity or interference between Phospholipase D2 (PLD2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.55ng/mL.
Intended UseHuman PLD2 ELISA Kit allows for the in vitro quantitative determination of PLD2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase D2 (PLD2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase D2 (PLD2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase D2 (PLD2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase D2 (PLD2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Phospholipase D2 Protein information
Uniprot ID PLD2_HUMAN
Uniprot AC O14939; I3L2C9; O43540; O43579; O43580; Q6PGR0; Q96BY3;
UniGene Hs.104519;
GeneID 5338
KEGG hsa:5338;
Human Phospholipase D2 Protein SEQUENCE
SEQUENCE 933 AA; 105987 MW; F25F6B73B45F57ED CRC64;
MTATPESLFP TGDELDSSQL QMESDEVDTL KEGEDPADRM HPFLAIYELQ
SLKVHPLVFA PGVPVTAQVV GTERYTSGSK VGTCTLYSVR LTHGDFSWTT
KKKYRHFQEL HRDLLRHKVL MSLLPLARFA VAYSPARDAG NREMPSLPRA
GPEGSTRHAA SKQKYLENYL NRLLTMSFYR NYHAMTEFLE VSQLSFIPDL
GRKGLEGMIR KRSGGHRVPG LTCCGRDQVC YRWSKRWLVV KDSFLLYMCL
ETGAISFVQL FDPGFEVQVG KRSTEARHGV RIDTSHRSLI LKCSSYRQAR
WWAQEITELA QGPGRDFLQL HRHDSYAPPR PGTLARWFVN GAGYFAAVAD
AILRAQEEIF ITDWWLSPEV YLKRPAHSDD WRLDIMLKRK AEEGVRVSIL
LFKEVELALG INSGYSKRAL MLLHPNIKVM RHPDQVTLWA HHEKLLVVDQ
VVAFLGGLDL AYGRWDDLHY RLTDLGDSSE SAASQPPTPR PDSPATPDLS
HNQFFWLGKD YSNLITKDWV QLDRPFEDFI DRETTPRMPW RDVGVVVHGL
PARDLARHFI QRWNFTKTTK AKYKTPTYPY LLPKSTSTAN QLPFTLPGGQ
CTTVQVLRSV DRWSAGTLEN SILNAYLHTI RESQHFLYIE NQFFISCSDG
RTVLNKVGDE IVDRILKAHK QGWCYRVYVL LPLLPGFEGD ISTGGGNSIQ
AILHFTYRTL CRGEYSILHR LKAAMGTAWR DYISICGLRT HGELGGHPVS
ELIYIHSKVL IADDRTVIIG SANINDRSLL GKRDSELAVL IEDTETEPSL
MNGAEYQAGR FALSLRKHCF GVILGANTRP DLDLRDPICD DFFQLWQDMA
ESNANIYEQI FRCLPSNATR SLRTLREYVA VEPLATVSPP LARSELTQVQ
GHLVHFPLKF LEDESLLPPL GSKEGMIPLE VWT
UCSC uc002fzc.4; human. [O14939-1];

Category

Human PLD2 ELISA Kit

Human PLD 2 ELISA Kit;Human hPLD2 ELISA Kit;Human choline phosphatase 2 ELISA Kit;Human PLD1C ELISA Kit;Human phosphatidylcholine-hydrolyzing phospholipase D2 ELISA Kit;Human phospholipase D2 ELISA Kit;