Human PLSCR2 ELISA Kit

Alternative name
Human PL scramblase 2 ELISA Kit;Human ca(2+)-dependent phospholipid scramblase 2 ELISA Kit;Human phospholipid scramblase 2 ELISA Kit;
Catalog
E029258
species
Human
GenePLSCR2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman PLSCR2 ELISA Kit allows for the in vitro quantitative determination of PLSCR2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the Assay: PLSCR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PLSCR2 antibody and an PLSCR2-HRP conjugate. The assay sample and buffer are incubated together with PLSCR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLSCR2 concentration since PLSCR2 from samples and PLSCR2-HRP conjugate compete for the anti-PLSCR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PLSCR2 from the sample, fewer sites are left to bind PLSCR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLSCR2 concentration in each sample is interpolated from this standard curve.
Human Phospholipid scramblase 2 Protein information
Uniprot ID PLS2_HUMAN
Uniprot AC Q9NRY7; B4DXC3; J3KR76; Q0VAQ1; Q6NSW9; Q7Z4L7;
UniGene Hs.744414;
GeneID 57047
KEGG hsa:57047;
Human Phospholipid scramblase 2 Protein SEQUENCE
SEQUENCE 297 AA; 33504 MW; 58F50C24AB438729 CRC64;
MRSWNSLFCL NSSRPPGHIV YPKHQAGHTG KQADHLGSQA FYPGRQHDYL
VPPAGTAGIP VQNQPGRPEG VPWMPAPPPP LNCPPGLEYL SQIDMILIHQ
QIELLEVLFS FESSNMYEIK NSFGQRIYFA AEDTNFCIRN CCGRSRPFTL
RITDNVGREV ITLERPLRCN CCCCPCCLQE IEIQAPPGVP VGYVTQTWHP
CLTKFTIKNQ KREDVLKISG PCIVCSCIAG VDFEITSLDE QIVVGRISKH
WSGFLREAFT DADNFGIQFP RDLDVKMKAV MIGACFLIDY MFFERTR
UCSC uc003evv.3; human. [Q9NRY7-2];

Category

Human PLSCR2 ELISA Kit

Human PL scramblase 2 ELISA Kit;Human ca(2+)-dependent phospholipid scramblase 2 ELISA Kit;Human phospholipid scramblase 2 ELISA Kit;