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Catalog
E029329
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species
Human
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GenePMLC
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PMLC. No significant cross-reactivity or interference between Human PMLC and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe limit of detection of Human PMLC defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 0.39 ng/mL (mean of 6 independent assays).
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Intended UseHuman PMLC ELISA Kit allows for the in vitro quantitative determination of PMLC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs a two-site sandwich ELISA to quantitative PMLC in Human serum, plasma. An antibody specific for PMLC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PMLC present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for PMLC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PMLC bound in the initial step. The color development is stopped and the intensity of the color is measured.
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