Human PIAS1 ELISA Kit

Alternative name
Human DEAD/H box-binding protein 1 ELISA Kit;Human gu-binding protein ELISA Kit;Human GBP ELISA Kit;Human protein inhibitor of activated STAT protein 1 ELISA Kit;Human RNA helicase II-binding protein ELISA Kit;Human DDXBP1 ELISA Kit;Human GU/RH-II ELISA Kit;Human ZMIZ3 ELISA Kit;Human protein inhibitor of activated STAT 1 ELISA Kit;Human E3 SUMO-protein ligase PIAS1 ELISA Kit;Human AR interacting protein ELISA Kit;Human DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 ELISA Kit;Human zinc finger, MIZ-type containing 3 ELISA Kit;
Catalog
E029375
species
Human
GenePIAS1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PIAS1. No significant cross-reactivity or interference between Human PIAS1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PIAS1 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman PIAS1 ELISA Kit allows for the in vitro quantitative determination of PIAS1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PIAS1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PIAS1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PIAS1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PIAS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PIAS1. You can calculate the concentration of Human PIAS1 in the samples by comparing the OD of the samples to the standard curve.
Human E3 SUMO-protein ligase PIAS1 Protein information
Uniprot ID PIAS1_HUMAN
Uniprot AC O75925; B2RB67; B3KSY9; C5J4B4; Q147X4; Q99751; Q9UN02;
UniGene Hs.162458; Hs.694796;
GeneID 8554
KEGG hsa:8554;
Human E3 SUMO-protein ligase PIAS1 Protein SEQUENCE
SEQUENCE 651 AA; 71836 MW; AA69338221124119 CRC64;
MADSAELKQM VMSLRVSELQ VLLGYAGRNK HGRKHELLTK ALHLLKAGCS
PAVQMKIKEL YRRRFPQKIM TPADLSIPNV HSSPMPATLS PSTIPQLTYD
GHPASSPLLP VSLLGPKHEL ELPHLTSALH PVHPDIKLQK LPFYDLLDEL
IKPTSLASDN SQRFRETCFA FALTPQQVQQ ISSSMDISGT KCDFTVQVQL
RFCLSETSCP QEDHFPPNLC VKVNTKPCSL PGYLPPTKNG VEPKRPSRPI
NITSLVRLST TVPNTIVVSW TAEIGRNYSM AVYLVKQLSS TVLLQRLRAK
GIRNPDHSRA LIKEKLTADP DSEIATTSLR VSLLCPLGKM RLTIPCRALT
CSHLQCFDAT LYIQMNEKKP TWVCPVCDKK APYEHLIIDG LFMEILKYCT
DCDEIQFKED GTWAPMRSKK EVQEVSASYN GVDGCLSSTL EHQVASHHQS
SNKNKKVEVI DLTIDSSSDE EEEEPSAKRT CPSLSPTSPL NNKGILSLPH
QASPVSRTPS LPAVDTSYIN TSLIQDYRHP FHMTPMPYDL QGLDFFPFLS
GDNQHYNTSL LAAAAAAVSD DQDLLHSSRF FPYTSSQMFL DQLSAGGSTS
LPTTNGSSSG SNSSLVSSNS LRESHSHTVT NRSSTDTASI FGIIPDIISL
D
UCSC uc002aqz.4; human. [O75925-1];

Category

Human PIAS1 ELISA Kit