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Alternative name
Pigment epithelium-derived factor ELISA KIT; Cell proliferation-inducing gene 35 protein ELISA KIT; EPC-1 ELISA KIT; Serpin F1SERPINF1 ELISA KIT; PEDF ELISA KIT; PEDF ELISA KIT
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Catalog
E029390
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species
Human
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GenePEDF
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Pigment Epithelium Derived Factor (PEDF).
No significant cross-reactivity or interference between Pigment Epithelium Derived Factor (PEDF) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.271ng/mL.
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Intended UseHuman PEDF ELISA Kit allows for the in vitro quantitative determination of PEDF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pigment Epithelium Derived Factor (PEDF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pigment Epithelium Derived Factor (PEDF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pigment Epithelium Derived Factor (PEDF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pigment Epithelium Derived Factor (PEDF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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