Human PKP2 ELISA Kit

Alternative name
Human ARVD9 ELISA Kit;Human plakophilin 2 ELISA Kit;Human plakophilin-2 ELISA Kit;
Catalog
E029551
species
Human
GenePKP2
SpecificityThis assay has high sensitivity and excellent specificity for detection of PKP2. No significant cross-reactivity or interference between PKP2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PKP2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman PKP2 ELISA Kit allows for the in vitro quantitative determination of PKP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: PKP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PKP2 antibody and an PKP2-HRP conjugate. The assay sample and buffer are incubated together with PKP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKP2 concentration since PKP2 from samples and PKP2-HRP conjugate compete for the anti-PKP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PKP2 from the sample, fewer sites are left to bind PKP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKP2 concentration in each sample is interpolated from this standard curve.
Human Plakophilin-2 Protein information
Uniprot ID PKP2_HUMAN
Uniprot AC Q99959; A0AV37; B8QFA1; B8QGS6; B8QGS7; D3DUW9; Q4VC01; Q99960;
UniGene Hs.164384;
GeneID 5318
KEGG hsa:5318;
Human Plakophilin-2 Protein SEQUENCE
SEQUENCE 881 AA; 97415 MW; 947838B0C8275D5D CRC64;
MAAPGAPAEY GYIRTVLGQQ ILGQLDSSSL ALPSEAKLKL AGSSGRGGQT
VKSLRIQEQV QQTLARKGRS SVGNGNLHRT SSVPEYVYNL HLVENDFVGG
RSPVPKTYDM LKAGTTATYE GRWGRGTAQY SSQKSVEERS LRHPLRRLEI
SPDSSPERAH YTHSDYQYSQ RSQAGHTLHH QESRRAALLV PPRYARSEIV
GVSRAGTTSR QRHFDTYHRQ YQHGSVSDTV FDSIPANPAL LTYPRPGTSR
SMGNLLEKEN YLTAGLTVGQ VRPLVPLQPV TQNRASRSSW HQSSFHSTRT
LREAGPSVAV DSSGRRAHLT VGQAAAGGSG NLLTERSTFT DSQLGNADME
MTLERAVSML EADHMLPSRI SAAATFIQHE CFQKSEARKR VNQLRGILKL
LQLLKVQNED VQRAVCGALR NLVFEDNDNK LEVAELNGVP RLLQVLKQTR
DLETKKQITD HTVNLRSRNG WPGAVAHACN PSTLGGQGGR ITRSGVRDQP
DQHGLLWNLS SNDKLKNLMI TEALLTLTEN IIIPFSGWPE GDYPKANGLL
DFDIFYNVTG CLRNMSSAGA DGRKAMRRCD GLIDSLVHYV RGTIADYQPD
DKATENCVCI LHNLSYQLEA ELPEKYSQNI YIQNRNIQTD NNKSIGCFGS
RSRKVKEQYQ DVPMPEEKSN PKGVEWLWHS IVIRMYLSLI AKSVRNYTQE
ASLGALQNLT AGSGPMPTSV AQTVVQKESG LQHTRKMLHV GDPSVKKTAI
SLLRNLSRNL SLQNEIAKET LPDLVSIIPD TVPSTDLLIE TTASACYTLN
NIIQNSYQNA RDLLNTGGIQ KIMAISAGDA YASNKASKAA SVLLYSLWAH
TELHHAYKKA QFKKTDFVNS RTAKAYHSLK D
UCSC uc001rlj.5; human. [Q99959-1];

Category

Human PKP2 ELISA Kit

Human ARVD9 ELISA Kit;Human plakophilin 2 ELISA Kit;Human plakophilin-2 ELISA Kit;