Human PLS1 ELISA Kit

Alternative name
Human Intestine-specific plastin ELISA Kit;Human I-plastin ELISA Kit;Human plastin 1 ELISA Kit;Human plastin-1 ELISA Kit;Human I plastin ELISA Kit;Human fimbrin ELISA Kit;Human intestine specific plastin ELISA Kit;
Catalog
E029622
species
Human
GenePLS1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Plastin 1 (PLS1). No significant cross-reactivity or interference between Plastin 1 (PLS1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.115ng/mL.
Intended UseHuman PLS1 ELISA Kit allows for the in vitro quantitative determination of PLS1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plastin 1 (PLS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Plastin 1 (PLS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Plastin 1 (PLS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plastin 1 (PLS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Plastin-1 Protein information
Uniprot ID PLSI_HUMAN
Uniprot AC Q14651; A8K2Q1; D3DNG3; Q8NEG6;
UniGene Hs.203637;
GeneID 5357
KEGG hsa:5357;
Human Plastin-1 Protein SEQUENCE
SEQUENCE 629 AA; 70253 MW; F904031933C2C323 CRC64;
MENSTTTISR EELEELQEAF NKIDIDNSGY VSDYELQDLF KEASLPLPGY
KVREIVEKIL SVADSNKDGK ISFEEFVSLM QELKSKDISK TFRKIINKRE
GITAIGGTST ISSEGTQHSY SEEEKVAFVN WINKALENDP DCKHLIPMNP
NDDSLFKSLA DGILLCKMIN LSEPDTIDER AINKKKLTPF TISENLNLAL
NSASAIGCTV VNIGASDLKE GKPHLVLGLL WQIIKVGLFA DIEISRNEAL
IALLNEGEEL EELMKLSPEE LLLRWVNYHL TNAGWHTISN FSQDIKDSRA
YFHLLNQIAP KGGEDGPAIA IDLSGINETN DLKRAGLMLQ EADKLGCKQF
VTPADVVSGN PKLNLAFVAN LFNTYPCLHK PNNNDIDMNL LEGESKEERT
FRNWMNSLGV NPYINHLYSD LADALVIFQL YEMIRVPVNW SHVNKPPYPA
LGGNMKKIEN CNYAVELGKN KAKFSLVGIA GQDLNEGNST LTLALVWQLM
RRYTLNVLSD LGEGEKVNDE IIIKWVNQTL KSANKKTSIS SFKDKSISTS
LPVLDLIDAI APNAVRQEMI RRENLSDEDK LNNAKYAISV ARKIGARIYA
LPDDLVEVKP KMVMTVFACL MGKGLNRIK
UCSC uc003euz.4; human.;

Category

Human PLS1 ELISA Kit

Human Intestine-specific plastin ELISA Kit;Human I-plastin ELISA Kit;Human plastin 1 ELISA Kit;Human plastin-1 ELISA Kit;Human I plastin ELISA Kit;Human fimbrin ELISA Kit;Human intestine specific plastin ELISA Kit;