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Human PAFAH2 ELISA Kit

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  • Alternative name

    Human serine-dependent phospholipase A2 ELISA Kit;Human SD-PLA2 ELISA Kit;Human HSD-PLA2 ELISA Kit;Human platelet activating factor acetylhydrolase 2 ELISA Kit;Human platelet-activating factor acetylhydrolase 2, cytoplasmic ELISA Kit;Human platelet-activating factor acetylhydrolase 2, 40kDa ELISA Kit;
  • Catalog
    E029633
  • species
    Human
  • GenePAFAH2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Platelet Activating Factor Acetylhydrolase 2 (PAFAH2). No significant cross-reactivity or interference between Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.105ng/mL.
  • Intended UseHuman PAFAH2 ELISA Kit allows for the in vitro quantitative determination of PAFAH2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Activating Factor Acetylhydrolase 2 (PAFAH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Activating Factor Acetylhydrolase 2 (PAFAH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Activating Factor Acetylhydrolase 2 (PAFAH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Platelet-activating factor acetylhydrolase 2, cytoplasmic Protein information
  • Uniprot ID PAFA2_HUMAN
  • Uniprot AC Q99487; D3DPK1; O15458; Q5SY02;
  • UniGene Hs.477083;
  • GeneID 5051
  • KEGG hsa:5051;
  • Human Platelet-activating factor acetylhydrolase 2, cytoplasmic Protein SEQUENCE

  • SEQUENCE 392 AA; 44036 MW; 690FB7E6F5B68317 CRC64;

    MGVNQSVGFP PVTGPHLVGC GDVMEGQNLQ GSFFRLFYPC QKAEETMEQP

    LWIPRYEYCT GLAEYLQFNK RCGGLLFNLA VGSCRLPVSW NGPFKTKDSG

    YPLIIFSHGL GAFRTLYSAF CMELASRGFV VAVPEHRDRS AATTYFCKQA

    PEENQPTNES LQEEWIPFRR VEEGEKEFHV RNPQVHQRVS ECLRVLKILQ

    EVTAGQTVFN ILPGGLDLMT LKGNIDMSRV AVMGHSFGGA TAILALAKET

    QFRCAVALDA WMFPLERDFY PKARGPVFFI NTEKFQTMES VNLMKKICAQ

    HEQSRIITVL GSVHRSQTDF AFVTGNLIGK FFSTETRGSL DPYEGQEVMV

    RAMLAFLQKH LDLKEDYNQW NNLIEGIGPS LTPGAPHHLS SL

  • UCSC uc001bld.4; human.;



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