Human PDGFRL ELISA Kit

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  • Alternative name

    Human PDGFR-like protein ELISA Kit;Human PDGF receptor beta-like tumor suppressor ELISA Kit;Human PRLTS ELISA Kit;Human PDGRL ELISA Kit;Human platelet derived growth factor receptor like ELISA Kit;Human platelet-derived growth factor receptor-like protein ELISA Kit;Human platelet-derived growth factor-beta-like tumor suppressor ELISA Kit;

  • Catalog
    E029679
  • species
    Human
  • GenePDGFRL
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PDGFRL ELISA Kit allows for the in vitro quantitative determination of PDGFRL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical
    Principle of the Assay: PDGFRL ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PDGFRL. Standards or samples are then added to the microtiter plate wells and PDGFRL if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PDGFRL present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PDGFRL are added to each well to "sandwich" the PDGFRL immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PDGFRL and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDGFRL concentration in each sample is interpolated from this standard curve.
  • Human Platelet-derived growth factor receptor-like protein Protein information
  • Uniprot ID PGFRL_HUMAN
  • Uniprot AC Q15198; A8K085; Q6FH04;
  • UniGene Hs.458573;
  • GeneID 5157
  • KEGG hsa:5157;
  • Human Platelet-derived growth factor receptor-like protein Protein SEQUENCE
  • SEQUENCE 375 AA; 41861 MW; DF7FE6EB3FB2A802 CRC64;

    MKVWLLLGLL LVHEALEDVT GQHLPKNKRP KEPGENRIKP TNKKVKPKIP

    KMKDRDSANS APKTQSIMMQ VLDKGRFQKP AATLSLLAGQ TVELRCKGSR

    IGWSYPAYLD TFKDSRLSVK QNERYGQLTL VNSTSADTGE FSCWVQLCSG

    YICRKDEAKT GSTYIFFTEK GELFVPSPSY FDVVYLNPDR QAVVPCRVTV

    LSAKVTLHRE FPAKEIPANG TDIVYDMKRG FVYLQPHSEH QGVVYCRAEA

    GGRSQISVKY QLLYVAVPSG PPSTTILASS NKVKSGDDIS VLCTVLGEPD

    VEVEFTWIFP GQKDERPVTI QDTWRLIHRG LGHTTRISQS VITVEDFETI

    DAGYYICTAQ NLQGQTTVAT TVEFS

  • UCSC uc003wxr.3; human.;



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