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Human PDGFB ELISA Kit

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  • Alternative name

    Human PDGF subunit B ELISA Kit;Human PDGF-2 ELISA Kit;Human platelet-derived growth factor B chain ELISA Kit;Human Platelet-derived growth factor beta polypeptide ELISA Kit;Human proto-oncogene c-Sis ELISA Kit;Human PDGF2 ELISA Kit;Human SIS ELISA Kit;Human IBGC5 ELISA Kit;Human SSV ELISA Kit;Human c-sis ELISA Kit;Human platelet derived growth factor subunit B ELISA Kit;Human platelet-derived growth factor subunit B ELISA Kit;Human PDGF, B chain ELISA Kit;Human becaplermin ELISA Kit;Human platelet-derived growth factor 2 ELISA Kit;Human platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) ELISA Kit;Human platelet-derived growth factor, beta polypeptide (oncogene SIS) ELISA Kit;
  • Catalog
    E029688
  • species
    Human
  • GenePDGFB
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor Subunit B (PDGFB). No significant cross-reactivity or interference between Platelet Derived Growth Factor Subunit B (PDGFB) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 6.3pg/mL.
  • Intended UseHuman PDGFB ELISA Kit allows for the in vitro quantitative determination of PDGFB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Subunit B (PDGFB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor Subunit B (PDGFB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Derived Growth Factor Subunit B (PDGFB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Platelet-derived growth factor subunit B Protein information
  • Uniprot ID PDGFB_HUMAN
  • Uniprot AC P01127; G3XAG8; P78431; Q15354; Q6FHE7; Q9UF23;
  • UniGene Hs.1976;
  • GeneID 5155
  • KEGG hsa:5155;
  • Human Platelet-derived growth factor subunit B Protein SEQUENCE

  • SEQUENCE 241 AA; 27283 MW; 9F9A3474CE203C0B CRC64;

    MNRCWALFLS LCCYLRLVSA EGDPIPEELY EMLSDHSIRS FDDLQRLLHG

    DPGEEDGAEL DLNMTRSHSG GELESLARGR RSLGSLTIAE PAMIAECKTR

    TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP

    VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT RSPGGSQEQR

    AKTPQTRVTI RTVRVRRPPK GKHRKFKHTH DKTALKETLG A

  • UCSC uc003axe.4; human. [P01127-1];



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