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Human PLEK ELISA Kit

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  • Alternative name

    Human platelet 47 kDa protein ELISA Kit;Human P47 ELISA Kit;Human pleckstrin ELISA Kit;
  • Catalog
    E029811
  • species
    Human
  • GenePLEK
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PLEK. No significant cross-reactivity or interference between Human PLEK and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe limit of detection of Human PLEK defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 0.078 ng/mL (mean of 6 independent assays).
  • Intended UseHuman PLEK ELISA Kit allows for the in vitro quantitative determination of PLEK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitative PLEK in Human serum, plasma. An antibody specific for PLEK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLEK present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for PLEK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLEK bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Pleckstrin Protein information
  • Uniprot ID PLEK_HUMAN
  • Uniprot AC P08567; B2R9E8; Q53SU8; Q6FGM8; Q6FGQ1; Q8WV81;
  • UniGene Hs.468840;
  • GeneID 5341
  • KEGG hsa:5341;
  • Human Pleckstrin Protein SEQUENCE

  • SEQUENCE 350 AA; 40125 MW; 2E292CB18B533261 CRC64;

    MEPKRIREGY LVKKGSVFNT WKPMWVVLLE DGIEFYKKKS DNSPKGMIPL

    KGSTLTSPCQ DFGKRMFVFK ITTTKQQDHF FQAAFLEERD AWVRDIKKAI

    KCIEGGQKFA RKSTRRSIRL PETIDLGALY LSMKDTEKGI KELNLEKDKK

    IFNHCFTGNC VIDWLVSNQS VRNRQEGLMI ASSLLNEGYL QPAGDMSKSA

    VDGTAENPFL DNPDAFYYFP DSGFFCEENS SDDDVILKEE FRGVIIKQGC

    LLKQGHRRKN WKVRKFILRE DPAYLHYYDP AGAEDPLGAI HLRGCVVTSV

    ESNSNGRKSE EENLFEIITA DEVHYFLQAA TPKERTEWIR AIQMASRTGK

  • UCSC uc002sen.5; human.;



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