Human ADAM10 ELISA Kit

Alternative name
Human ADAM 10 ELISA Kit;Human CDw156 ELISA Kit;Human kuzbanian protein homolog ELISA Kit;Human mammalian disintegrin-metalloprotease ELISA Kit;Human kuz ELISA Kit;Human MADM ELISA Kit;Human AD10 ELISA Kit;Human AD18 ELISA Kit;Human CD156c ELISA Kit;Human HsT18717 ELISA Kit;Human RAK ELISA Kit;Human ADAM metallopeptidase domain 10 ELISA Kit;Human disintegrin and metalloproteinase domain-containing protein 10 ELISA Kit;Human a disintegrin and metalloprotease domain 10 ELISA Kit;
Catalog
E000003
species
Human
GeneADAM10
SpecificityThis assay has high sensitivity and excellent specificity for detection of ADAM10. No significant cross-reactivity or interference between ADAM10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAM10 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman ADAM10 ELISA Kit allows for the in vitro quantitative determination of ADAM10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalIntended Uses: This ADAM10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADAM10. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ADAM10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ADAM10 antibody and an ADAM10-HRP conjugate. The assay sample and buffer are incubated together with ADAM10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM10 concentration since ADAM10 from samples and ADAM10-HRP conjugate compete for the anti-ADAM10 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM10 from the sample, fewer sites are left to bind ADAM10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM10 concentration in each sample is interpolated from this standard curve.
Human Disintegrin and metalloproteinase domain-containing protein 10 Protein information
Uniprot ID ADA10_HUMAN
Uniprot AC O14672; B4DU28; Q10742; Q92650;
UniGene Hs.172028; Hs.578508; Hs.745136;
GeneID 102
KEGG hsa:102;
Human Disintegrin and metalloproteinase domain-containing protein 10 Protein SEQUENCE
SEQUENCE 748 AA; 84142 MW; 0881E65B17022A71 CRC64;
MVLLRVLILL LSWAAGMGGQ YGNPLNKYIR HYEGLSYNVD SLHQKHQRAK
RAVSHEDQFL RLDFHAHGRH FNLRMKRDTS LFSDEFKVET SNKVLDYDTS
HIYTGHIYGE EGSFSHGSVI DGRFEGFIQT RGGTFYVEPA ERYIKDRTLP
FHSVIYHEDD INYPHKYGPQ GGCADHSVFE RMRKYQMTGV EEVTQIPQEE
HAANGPELLR KKRTTSAEKN TCQLYIQTDH LFFKYYGTRE AVIAQISSHV
KAIDTIYQTT DFSGIRNISF MVKRIRINTT ADEKDPTNPF RFPNIGVEKF
LELNSEQNHD DYCLAYVFTD RDFDDGVLGL AWVGAPSGSS GGICEKSKLY
SDGKKKSLNT GIITVQNYGS HVPPKVSHIT FAHEVGHNFG SPHDSGTECT
PGESKNLGQK ENGNYIMYAR ATSGDKLNNN KFSLCSIRNI SQVLEKKRNN
CFVESGQPIC GNGMVEQGEE CDCGYSDQCK DECCFDANQP EGRKCKLKPG
KQCSPSQGPC CTAQCAFKSK SEKCRDDSDC AREGICNGFT ALCPASDPKP
NFTDCNRHTQ VCINGQCAGS ICEKYGLEEC TCASSDGKDD KELCHVCCMK
KMDPSTCAST GSVQWSRHFS GRTITLQPGS PCNDFRGYCD VFMRCRLVDA
DGPLARLKKA IFSPELYENI AEWIVAHWWA VLLMGIALIM LMAGFIKICS
VHTPSSNPKL PPPKPLPGTL KRRRPPQPIQ QPQRQRPRES YQMGHMRR
UCSC uc002afd.3; human. [O14672-1];

Category

Human ADAM10 ELISA Kit