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Human HCN2 ELISA Kit

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  • Alternative name

    Human brain cyclic nucleotide-gated channel 2 ELISA Kit;Human BCNG-2 ELISA Kit;Human BCNG2 ELISA Kit;Human HAC-1 ELISA Kit;Human hyperpolarization activated cyclic nucleotide gated potassium channel 2 ELISA Kit;Human potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 ELISA Kit;
  • Catalog
    E030328
  • species
    Human
  • GeneHCN2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human HCN2. No significant cross-reactivity or interference between Human HCN2 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe limit of detection of Human HCN2 defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 0.1 ng/mL (mean of 6 independent assays).
  • Intended UseHuman HCN2 ELISA Kit allows for the in vitro quantitative determination of HCN2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs a two-site sandwich ELISA to quantitative HCN2 in Human serum, plasma. An antibody specific for HCN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HCN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for HCN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HCN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 Protein information
  • Uniprot ID HCN2_HUMAN
  • Uniprot AC Q9UL51; O60742; O60743; O75267; Q9UBS2;
  • UniGene Hs.124161;
  • GeneID 610
  • KEGG hsa:610;
  • Human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 Protein SEQUENCE

  • SEQUENCE 889 AA; 96950 MW; 4B263E0C06C2A47D CRC64;

    MDARGGGGRP GESPGATPAP GPPPPPPPAP PQQQPPPPPP PAPPPGPGPA

    PPQHPPRAEA LPPEAADEGG PRGRLRSRDS SCGRPGTPGA ASTAKGSPNG

    ECGRGEPQCS PAGPEGPARG PKVSFSCRGA ASGPAPGPGP AEEAGSEEAG

    PAGEPRGSQA SFMQRQFGAL LQPGVNKFSL RMFGSQKAVE REQERVKSAG

    AWIIHPYSDF RFYWDFTMLL FMVGNLIIIP VGITFFKDET TAPWIVFNVV

    SDTFFLMDLV LNFRTGIVIE DNTEIILDPE KIKKKYLRTW FVVDFVSSIP

    VDYIFLIVEK GIDSEVYKTA RALRIVRFTK ILSLLRLLRL SRLIRYIHQW

    EEIFHMTYDL ASAVMRICNL ISMMLLLCHW DGCLQFLVPM LQDFPRNCWV

    SINGMVNHSW SELYSFALFK AMSHMLCIGY GRQAPESMTD IWLTMLSMIV

    GATCYAMFIG HATALIQSLD SSRRQYQEKY KQVEQYMSFH KLPADFRQKI

    HDYYEHRYQG KMFDEDSILG ELNGPLREEI VNFNCRKLVA SMPLFANADP

    NFVTAMLTKL KFEVFQPGDY IIREGTIGKK MYFIQHGVVS VLTKGNKEMK

    LSDGSYFGEI CLLTRGRRTA SVRADTYCRL YSLSVDNFNE VLEEYPMMRR

    AFETVAIDRL DRIGKKNSIL LHKVQHDLNS GVFNNQENAI IQEIVKYDRE

    MVQQAELGQR VGLFPPPPPP PQVTSAIATL QQAAAMSFCP QVARPLVGPL

    ALGSPRLVRR PPPGPAPAAA SPGPPPPASP PGAPASPRAP RTSPYGGLPA

    APLAGPALPA RRLSRASRPL SASQPSLPHG APGPAASTRP ASSSTPRLGP

    TPAARAAAPS PDRRDSASPG AAGGLDPQDS ARSRLSSNL

  • UCSC uc002lpe.3; human.;



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