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Human PRAME ELISA Kit

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  • Alternative name

    Human opa-interacting protein 4 ELISA Kit;Human OIP-4 ELISA Kit;Human preferentially expressed antigen of melanoma ELISA Kit;Human MAPE ELISA Kit;Human OIP4 ELISA Kit;Human CT130 ELISA Kit;Human preferentially expressed antigen in melanoma ELISA Kit;Human melanoma antigen preferentially expressed in tumors ELISA Kit;Human Opa-interacting protein OIP4 ELISA Kit;Human cancer/testis antigen 130 ELISA Kit;
  • Catalog
    E030486
  • species
    Human
  • GenePRAME
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Preferentially Expressed Antigen In Melanoma (PRAME). No significant cross-reactivity or interference between Preferentially Expressed Antigen In Melanoma (PRAME) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.117ng/mL.
  • Intended UseHuman PRAME ELISA Kit allows for the in vitro quantitative determination of PRAME , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Preferentially Expressed Antigen In Melanoma (PRAME). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Preferentially Expressed Antigen In Melanoma (PRAME). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Preferentially Expressed Antigen In Melanoma (PRAME), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Preferentially Expressed Antigen In Melanoma (PRAME) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Melanoma antigen preferentially expressed in tumors Protein information
  • Uniprot ID PRAME_HUMAN
  • Uniprot AC P78395; B2R6Y7; O43481; Q8IXN8;
  • UniGene Hs.30743;
  • GeneID 23532
  • KEGG hsa:23532;
  • Human Melanoma antigen preferentially expressed in tumors Protein SEQUENCE

  • SEQUENCE 509 AA; 57890 MW; B5FFF3E7F7E82606 CRC64;

    MERRRLWGSI QSRYISMSVW TSPRRLVELA GQSLLKDEAL AIAALELLPR

    ELFPPLFMAA FDGRHSQTLK AMVQAWPFTC LPLGVLMKGQ HLHLETFKAV

    LDGLDVLLAQ EVRPRRWKLQ VLDLRKNSHQ DFWTVWSGNR ASLYSFPEPE

    AAQPMTKKRK VDGLSTEAEQ PFIPVEVLVD LFLKEGACDE LFSYLIEKVK

    RKKNVLRLCC KKLKIFAMPM QDIKMILKMV QLDSIEDLEV TCTWKLPTLA

    KFSPYLGQMI NLRRLLLSHI HASSYISPEK EEQYIAQFTS QFLSLQCLQA

    LYVDSLFFLR GRLDQLLRHV MNPLETLSIT NCRLSEGDVM HLSQSPSVSQ

    LSVLSLSGVM LTDVSPEPLQ ALLERASATL QDLVFDECGI TDDQLLALLP

    SLSHCSQLTT LSFYGNSISI SALQSLLQHL IGLSNLTHVL YPVPLESYED

    IHGTLHLERL AYLHARLREL LCELGRPSMV WLSANPCPHC GDRTFYDPEP

    ILCPCFMPN

  • UCSC uc032qia.2; human.;



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