Human PSG1 ELISA Kit

Alternative name
Human PS-beta-G-1 ELISA Kit;Human PSBG-1 ELISA Kit;Human Pregnancy-specific glycoprotein 1 ELISA Kit;Human CD66 antigen-like family member F ELISA Kit;Human fetal liver non-specific cross-reactive antigen 1/2 ELISA Kit;Human FL-NCA-1/2 ELISA Kit;Human PSG95 ELISA Kit;Human pregnancy-specific beta-1 glycoprotein C/D ELISA Kit;Human PS-beta-C/D ELISA Kit;Human B1G1 ELISA Kit;Human PSBG1 ELISA Kit;Human PSGGA ELISA Kit;Human CD66f ELISA Kit;Human DHFRP2 ELISA Kit;Human PBG1 ELISA Kit;Human PSGIIA ELISA Kit;Human SP1 ELISA Kit;Human pregnancy specific beta-1-glycoprotein 1 ELISA Kit;Human pregnancy-specific beta-1-glycoprotein 1 ELISA Kit;Human pregnancy-specific B-1 glycoprotein ELISA Kit;
Catalog
E030525
species
Human
GenePSG1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman PSG1 ELISA Kit allows for the in vitro quantitative determination of PSG1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate 1. INTENDED USE This PS?1G ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PS?1G. This ELISA kit for research use only, not for therapeutic or diagnostic applications! 2. PRINCIPLE OF THE ASSAY PS?1G ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PS?1G antibody and an PS?1G-HRP conjugate. The assay sample and buffer are incubated together with PS?1G-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PS?1G concentration since PS?1G from samples and PS?1G-HRP conjugate compete for the anti-PS?1G antibody binding site. Since the number of sites is limited, as more sites are occupied by PS?1G from the sample, fewer sites are left to bind PS?1G-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PS?1G concentration in each sample is interpolated from this standard curve.
Human Pregnancy-specific beta-1-glycoprotein 1 Protein information
Uniprot ID PSG1_HUMAN
Uniprot AC Q6ICR4; Q9P1W5; Q9UQ79;
UniGene Hs.709192;
GeneID 5669
KEGG hsa:5669;
Human Pregnancy-specific beta-1-glycoprotein 1 Protein SEQUENCE
SEQUENCE 419 AA; 47223 MW; 5E9855162F0C796D CRC64;
MGTLSAPPCT QRIKWKGLLL TASLLNFWNL PTTAQVTIEA EPTKVSEGKD
VLLLVHNLPQ NLTGYIWYKG QMRDLYHYIT SYVVDGEIII YGPAYSGRET
AYSNASLLIQ NVTREDAGSY TLHIIKGDDG TRGVTGRFTF TLHLETPKPS
ISSSNLNPRE TMEAVSLTCD PETPDASYLW WMNGQSLPMT HSLKLSETNR
TLFLLGVTKY TAGPYECEIR NPVSASRSDP VTLNLLPKLP KPYITINNLN
PRENKDVLNF TCEPKSENYT YIWWLNGQSL PVSPRVKRPI ENRILILPSV
TRNETGPYQC EIRDRYGGIR SDPVTLNVLY GPDLPRIYPS FTYYRSGEVL
YLSCSADSNP PAQYSWTINE KFQLPGQKLF IRHITTKHSG LYVCSVRNSA
TGKESSKSMT VEVSDWTVP
UCSC uc002ouz.3; human. [P11464-1];

Category

Human PSG1 ELISA Kit