Human TRUB1 ELISA Kit

Alternative name
Human PUS4 ELISA Kit;Human TruB pseudouridine synthase family member 1 ELISA Kit;Human probable tRNA pseudouridine synthase 1 ELISA Kit;Human TruB pseudouridine (psi) synthase family member 1 ELISA Kit;Human TruB pseudouridine (psi) synthase homolog 1 ELISA Kit;
Catalog
E030871
species
Human
GeneTRUB1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<46.9pg/ml
Intended UseHuman TRUB1 ELISA Kit allows for the in vitro quantitative determination of TRUB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TRUB1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TRUB1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TRUB1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TRUB1 can be calculated.
Human Probable tRNA pseudouridine synthase 1 Protein information
Uniprot ID TRUB1_HUMAN
Uniprot AC Q8WWH5; B2R716; Q53ES2;
UniGene Hs.21187;
GeneID 142940
KEGG hsa:142940;
Human Probable tRNA pseudouridine synthase 1 Protein SEQUENCE
SEQUENCE 349 AA; 37253 MW; C3292D31EC60EE17 CRC64;
MAASEAAVVS SPSLKTDTSP VLETAGTVAA MAATPSARAA AAVVAAAART
GSEARVSKAA LATKLLSLSG VFAVHKPKGP TSAELLNRLK EKLLAEAGMP
SPEWTKRKKQ TLKIGHGGTL DSAARGVLVV GIGSGTKMLT SMLSGSKRYT
AIGELGKATD TLDSTGRVTE EKPYDKITQE DIEGILQKFT GNIMQVPPLY
SALKKDGQRL STLMKRGEVV EAKPARPVTV YSISLQKFQP PFFTLDVECG
GGFYIRSLVS DIGKELSSCA NVLELTRTKQ GPFTLEEHAL PEDKWTIDDI
AQSLEHCSSL FPAELALKKS KPESNEQVLS CEYITLNEPK REDDVIKTC
UCSC uc001lcd.4; human.;

Category

Human TRUB1 ELISA Kit

Human PUS4 ELISA Kit;Human TruB pseudouridine synthase family member 1 ELISA Kit;Human probable tRNA pseudouridine synthase 1 ELISA Kit;Human TruB pseudouridine (psi) synthase family member 1 ELISA Kit;Human TruB pseudouridine (psi) synthase homolog 1 ELISA Kit;