Human PHB ELISA Kit

Alternative name
Human HEL-215 ELISA Kit;Human HEL-S-54e ELISA Kit;Human PHB1 ELISA Kit;Human prohibitin ELISA Kit;Human epididymis luminal protein 215 ELISA Kit;Human epididymis secretory sperm binding protein Li 54e ELISA Kit;
Catalog
E031034
species
Human
GenePHB
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman PHB ELISA Kit allows for the in vitro quantitative determination of PHB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalIntended Uses: This PHB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PHB. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: PHB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PHB antibody and an PHB-HRP conjugate. The assay sample and buffer are incubated together with PHB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PHB concentration since PHB from samples and PHB-HRP conjugate compete for the anti-PHB antibody binding site. Since the number of sites is limited, as more sites are occupied by PHB from the sample, fewer sites are left to bind PHB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PHB concentration in each sample is interpolated from this standard curve.
Human Prohibitin Protein information
Uniprot ID PHB_HUMAN
Uniprot AC P35232; B4DY47; Q4VBQ0;
UniGene Hs.514303;
GeneID 5245
KEGG hsa:5245;
Human Prohibitin Protein SEQUENCE
SEQUENCE 272 AA; 29804 MW; 915494273E342C76 CRC64;
MAAKVFESIG KFGLALAVAG GVVNSALYNV DAGHRAVIFD RFRGVQDIVV
GEGTHFLIPW VQKPIIFDCR SRPRNVPVIT GSKDLQNVNI TLRILFRPVA
SQLPRIFTSI GEDYDERVLP SITTEILKSV VARFDAGELI TQRELVSRQV
SDDLTERAAT FGLILDDVSL THLTFGKEFT EAVEAKQVAQ QEAERARFVV
EKAEQQKKAA IISAEGDSKA AELIANSLAT AGDGLIELRK LEAAEDIAYQ
LSRSRNITYL PAGQSVLLQL PQ
UCSC uc002iox.3; human. [P35232-1];

Category

Human PHB ELISA Kit

Human HEL-215 ELISA Kit;Human HEL-S-54e ELISA Kit;Human PHB1 ELISA Kit;Human prohibitin ELISA Kit;Human epididymis luminal protein 215 ELISA Kit;Human epididymis secretory sperm binding protein Li 54e ELISA Kit;