-
Alternative name
Human endocrine-gland-derived vascular endothelial growth factor ELISA Kit;Human EG-VEGF ELISA Kit;Human mambakine ELISA Kit;Human EGVEGF ELISA Kit;Human PK1 ELISA Kit;Human PRK1 ELISA Kit;Human prokineticin 1 ELISA Kit;Human prokineticin-1 ELISA Kit;Human black mamba toxin-related protein ELISA Kit;
-
Catalog
E031051
-
species
Human
-
GenePROK1
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PROK1. No significant cross-reactivity or interference between Human PROK1 and analogues was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity0.078 ng/ml
-
Intended UseHuman PROK1 ELISA Kit allows for the in vitro quantitative determination of PROK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageStore the whole ELISA kit at 4℃
-
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EG-VEGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EG-VEGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain EG-VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of EG-VEGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-
Human Prokineticin-1 Protein information
-
Uniprot ID
PROK1_HUMAN
-
Uniprot AC
P58294; Q5VWD4; Q8TC69;
-
UniGene
Hs.514793;
-
GeneID
84432
-
KEGG
hsa:84432;
-
Human Prokineticin-1 Protein SEQUENCE
-
SEQUENCE 105 AA; 11715 MW; C7E3FDE30EFB416A CRC64;
MRGATRVSIM LLLVTVSDCA VITGACERDV QCGAGTCCAI SLWLRGLRMC
TPLGREGEEC
HPGSHKVPFF RKRKHHTCPC LPNLLCSRFP DGRYRCSMDL
KNINF
-
UCSC
uc001dzs.4; human.;
-