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Human P4HA1 ELISA Kit

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  • Alternative name

    Human 4-PH alpha-1 ELISA Kit;Human procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1 ELISA Kit;Human P4HA ELISA Kit;Human prolyl 4-hydroxylase subunit alpha 1 ELISA Kit;Human prolyl 4-hydroxylase subunit alpha-1 ELISA Kit;Human C-P4Halpha(I) ELISA Kit;Human collagen prolyl 4-hydroxylase alpha(I) ELISA Kit;Human procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I ELISA Kit;Human prolyl 4-hydroxylase, alpha polypeptide I ELISA Kit;
  • Catalog
    E031151
  • species
    Human
  • GeneP4HA1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.375ng/ml
  • Intended UseHuman P4HA1 ELISA Kit allows for the in vitro quantitative determination of P4HA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human P4HA1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human P4HA1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human P4HA1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman P4HA1can be calculated.
  • Human Prolyl 4-hydroxylase subunit alpha-1 Protein information
  • Uniprot ID P4HA1_HUMAN
  • Uniprot AC P13674; C9JL12; Q15082; Q15083; Q5VSQ5;
  • UniGene Hs.500047; Hs.593005;
  • GeneID 5033
  • KEGG hsa:5033;
  • Human Prolyl 4-hydroxylase subunit alpha-1 Protein SEQUENCE

  • SEQUENCE 534 AA; 61049 MW; EBAFA8CCF09A1DDB CRC64;

    MIWYILIIGI LLPQSLAHPG FFTSIGQMTD LIHTEKDLVT SLKDYIKAEE

    DKLEQIKKWA EKLDRLTSTA TKDPEGFVGH PVNAFKLMKR LNTEWSELEN

    LVLKDMSDGF ISNLTIQRQY FPNDEDQVGA AKALLRLQDT YNLDTDTISK

    GNLPGVKHKS FLTAEDCFEL GKVAYTEADY YHTELWMEQA LRQLDEGEIS

    TIDKVSVLDY LSYAVYQQGD LDKALLLTKK LLELDPEHQR ANGNLKYFEY

    IMAKEKDVNK SASDDQSDQK TTPKKKGVAV DYLPERQKYE MLCRGEGIKM

    TPRRQKKLFC RYHDGNRNPK FILAPAKQED EWDKPRIIRF HDIISDAEIE

    IVKDLAKPRL RRATISNPIT GDLETVHYRI SKSAWLSGYE NPVVSRINMR

    IQDLTGLDVS TAEELQVANY GVGGQYEPHF DFARKDEPDA FKELGTGNRI

    ATWLFYMSDV SAGGATVFPE VGASVWPKKG TAVFWYNLFA SGEGDYSTRH

    AACPVLVGNK WVSNKWLHER GQEFRRPCTL SELE

  • UCSC uc001jtg.4; human. [P13674-1];



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