Human P4HB ELISA Kit

Alternative name
Human PDI ELISA Kit;Human cellular thyroid hormone-binding protein ELISA Kit;Human prolyl 4-hydroxylase subunit beta ELISA Kit;Human p55 ELISA Kit;Human ERBA2L ELISA Kit;Human PDIA1 ELISA Kit;Human PO4DB ELISA Kit;Human CLCRP1 ELISA Kit;Human DSI ELISA Kit;Human GIT ELISA Kit;Human P4Hbeta ELISA Kit;Human PHDB ELISA Kit;Human PO4HB ELISA Kit;Human PROHB ELISA Kit;Human protein disulfide-isomerase ELISA Kit;Human collagen prolyl 4-hydroxylase beta ELISA Kit;Human glutathione-insulin transhydrogenase ELISA Kit;Human procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide ELISA Kit;Human prolyl 4-hydroxylase, beta polypeptide ELISA Kit;Human protein disulfide isomerase family A, member 1 ELISA Kit;Human protein disulfide isomerase-associated 1 ELISA Kit;Human protein disulfide isomerase/oxidoreductase ELISA Kit;Human protocollagen hydroxylase ELISA Kit;Human testicular secretory protein Li 32 ELISA Kit;Human thyroid hormone-binding protein p55 ELISA Kit;
Catalog
E031157
species
Human
GeneP4HB
SpecificityThis assay has high sensitivity and excellent specificity for detection of human PDI. No significant cross-reactivity or interference between human PDI and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human PDI is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman P4HB ELISA Kit allows for the in vitro quantitative determination of P4HB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDI is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDI bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Protein disulfide-isomerase Protein information
Uniprot ID PDIA1_HUMAN
Uniprot AC P07237; B2RDQ2; P30037; P32079; Q15205; Q6LDE5;
UniGene Hs.464336;
GeneID 5034
KEGG hsa:5034;
Human Protein disulfide-isomerase Protein SEQUENCE
SEQUENCE 508 AA; 57116 MW; 906CE6D9900B8FCE CRC64;
MLRRALLCLA VAALVRADAP EEEDHVLVLR KSNFAEALAA HKYLLVEFYA
PWCGHCKALA PEYAKAAGKL KAEGSEIRLA KVDATEESDL AQQYGVRGYP
TIKFFRNGDT ASPKEYTAGR EADDIVNWLK KRTGPAATTL PDGAAAESLV
ESSEVAVIGF FKDVESDSAK QFLQAAEAID DIPFGITSNS DVFSKYQLDK
DGVVLFKKFD EGRNNFEGEV TKENLLDFIK HNQLPLVIEF TEQTAPKIFG
GEIKTHILLF LPKSVSDYDG KLSNFKTAAE SFKGKILFIF IDSDHTDNQR
ILEFFGLKKE ECPAVRLITL EEEMTKYKPE SEELTAERIT EFCHRFLEGK
IKPHLMSQEL PEDWDKQPVK VLVGKNFEDV AFDEKKNVFV EFYAPWCGHC
KQLAPIWDKL GETYKDHENI VIAKMDSTAN EVEAVKVHSF PTLKFFPASA
DRTVIDYNGE RTLDGFKKFL ESGGQDGAGD DDDLEDLEEA EEPDMEEDDD
QKAVKDEL
UCSC uc002kbn.2; human.;

Category

Human P4HB ELISA Kit