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Human PCCB ELISA Kit

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  • Alternative name

    Human PCCase subunit beta ELISA Kit;Human propanoyl-CoA:carbon dioxide ligase subunit beta ELISA Kit;Human propionyl-CoA carboxylase beta subunit ELISA Kit;Human propionyl-CoA carboxylase beta chain, mitochondrial ELISA Kit;Human propionyl CoA carboxylase, beta polypeptide ELISA Kit;Human propionyl Coenzyme A carboxylase, beta polypeptide ELISA Kit;
  • Catalog
    E031193
  • species
    Human
  • GenePCCB
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PCCB. No significant cross-reactivity or interference between PCCB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PCCB and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PCCB ELISA Kit allows for the in vitro quantitative determination of PCCB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: This PCCB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PCCB. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||PCCB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PCCB antibody and an PCCB-HRP conjugate. The assay sample and buffer are incubated together with PCCB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PCCB concentration since PCCB from samples and PCCB-HRP conjugate compete for the anti-PCCB antibody binding site. Since the number of sites is limited, as more sites are occupied by PCCB from the sample, fewer sites are left to bind PCCB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCCB concentration in each sample is interpolated from this standard curve.
  • Human Propionyl-CoA carboxylase beta chain, mitochondrial Protein information
  • Uniprot ID PCCB_HUMAN
  • Uniprot AC P05166; B7Z2Z4; Q16813; Q96CX0;
  • UniGene Hs.63788;
  • GeneID 5096
  • KEGG hsa:5096;
  • Human Propionyl-CoA carboxylase beta chain, mitochondrial Protein SEQUENCE

  • SEQUENCE 539 AA; 58216 MW; A2DAAC00312D3C0F CRC64;

    MAAALRVAAV GARLSVLASG LRAAVRSLCS QATSVNERIE NKRRTALLGG

    GQRRIDAQHK RGKLTARERI SLLLDPGSFV ESDMFVEHRC ADFGMAADKN

    KFPGDSVVTG RGRINGRLVY VFSQDFTVFG GSLSGAHAQK ICKIMDQAIT

    VGAPVIGLND SGGARIQEGV ESLAGYADIF LRNVTASGVI PQISLIMGPC

    AGGAVYSPAL TDFTFMVKDT SYLFITGPDV VKSVTNEDVT QEELGGAKTH

    TTMSGVAHRA FENDVDALCN LRDFFNYLPL SSQDPAPVRE CHDPSDRLVP

    ELDTIVPLES TKAYNMVDII HSVVDEREFF EIMPNYAKNI IVGFARMNGR

    TVGIVGNQPK VASGCLDINS SVKGARFVRF CDAFNIPLIT FVDVPGFLPG

    TAQEYGGIIR HGAKLLYAFA EATVPKVTVI TRKAYGGAYD VMSSKHLCGD

    TNYAWPTAEI AVMGAKGAVE IIFKGHENVE AAQAEYIEKF ANPFPAAVRG

    FVDDIIQPSS TRARICCDLD VLASKKVQRP WRKHANIPL

  • UCSC uc003eqy.3; human. [P05166-1];



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