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Human PCSK1 ELISA Kit

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  • Alternative name

    Human NEC 1 ELISA Kit;Human prohormone convertase 1 ELISA Kit;Human Proprotein convertase 1 ELISA Kit;Human PC1 ELISA Kit;Human NEC1 ELISA Kit;Human BMIQ12 ELISA Kit;Human PC3 ELISA Kit;Human SPC3 ELISA Kit;Human proprotein convertase subtilisin/kexin type 1 ELISA Kit;Human neuroendocrine convertase 1 ELISA Kit;Human prohormone convertase 3 ELISA Kit;
  • Catalog
    E031197
  • species
    Human
  • GenePCSK1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1). No significant cross-reactivity or interference between Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.101ng/mL.
  • Intended UseHuman PCSK1 ELISA Kit allows for the in vitro quantitative determination of PCSK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Neuroendocrine convertase 1 Protein information
  • Uniprot ID NEC1_HUMAN
  • Uniprot AC P29120; B7Z8T7; E9PHA1; P78478; Q92532;
  • UniGene Hs.78977;
  • GeneID 5122
  • KEGG hsa:5122;
  • Human Neuroendocrine convertase 1 Protein SEQUENCE

  • SEQUENCE 753 AA; 84152 MW; D3CBD1B92093A208 CRC64;

    MERRAWSLQC TAFVLFCAWC ALNSAKAKRQ FVNEWAAEIP GGPEAASAIA

    EELGYDLLGQ IGSLENHYLF KHKNHPRRSR RSAFHITKRL SDDDRVIWAE

    QQYEKERSKR SALRDSALNL FNDPMWNQQW YLQDTRMTAA LPKLDLHVIP

    VWQKGITGKG VVITVLDDGL EWNHTDIYAN YDPEASYDFN DNDHDPFPRY

    DPTNENKHGT RCAGEIAMQA NNHKCGVGVA YNSKVGGIRM LDGIVTDAIE

    ASSIGFNPGH VDIYSASWGP NDDGKTVEGP GRLAQKAFEY GVKQGRQGKG

    SIFVWASGNG GRQGDNCDCD GYTDSIYTIS ISSASQQGLS PWYAEKCSST

    LATSYSSGDY TDQRITSADL HNDCTETHTG TSASAPLAAG IFALALEANP

    NLTWRDMQHL VVWTSEYDPL ANNPGWKKNG AGLMVNSRFG FGLLNAKALV

    DLADPRTWRS VPEKKECVVK DNDFEPRALK ANGEVIIEIP TRACEGQENA

    IKSLEHVQFE ATIEYSRRGD LHVTLTSAAG TSTVLLAERE RDTSPNGFKN

    WDFMSVHTWG ENPIGTWTLR ITDMSGRIQN EGRIVNWKLI LHGTSSQPEH

    MKQPRVYTSY NTVQNDRRGV EKMVDPGEEQ PTQENPKENT LVSKSPSSSS

    VGGRRDELEE GAPSQAMLRL LQSAFSKNSP PKQSPKKSPS AKLNIPYENF

    YEALEKLNKP SQLKDSEDSL YNDYVDVFYN TKPYKHRDDR LLQALVDILN

    EEN

  • UCSC uc003kls.3; human. [P29120-1];



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