Human PTGIS ELISA Kit

Alternative name
Human prostaglandin I2 synthase ELISA Kit;Human CYP8 ELISA Kit;Human CYP8A1 ELISA Kit;Human PGIS ELISA Kit;Human PTGI ELISA Kit;Human prostacyclin synthase ELISA Kit;Human cytochrome P450, family 8, subfamily A, polypeptide 1 ELISA Kit;Human prostaglandin I2 (prostacyclin) synthase ELISA Kit;
Catalog
E031316
species
Human
GenePTGIS
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman PTGIS ELISA Kit allows for the in vitro quantitative determination of PTGIS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This PTGIS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PTGIS. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAYPTGIS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTGIS antibody and an PTGIS-HRP conjugate. The assay sample and buffer are incubated together with PTGIS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTGIS concentration since PTGIS from samples and PTGIS-HRP conjugate compete for the anti-PTGIS antibody binding site. Since the number of sites is limited, as more sites are occupied by PTGIS from the sample, fewer sites are left to bind PTGIS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTGIS concentration in each sample is interpolated from this standard curve
Human Prostacyclin synthase Protein information
Uniprot ID PTGIS_HUMAN
Uniprot AC Q16647; Q3MII8; Q9HAX2; Q9HAX3; Q9HAX4;
UniGene Hs.302085;
GeneID 5740
KEGG hsa:5740;
Human Prostacyclin synthase Protein SEQUENCE
SEQUENCE 500 AA; 57104 MW; 39595442BFC0B625 CRC64;
MAWAALLGLL AALLLLLLLS RRRTRRPGEP PLDLGSIPWL GYALDFGKDA
ASFLTRMKEK HGDIFTILVG GRYVTVLLDP HSYDAVVWEP RTRLDFHAYA
IFLMERIFDV QLPHYSPSDE KARMKLTLLH RELQALTEAM YTNLHAVLLG
DATEAGSGWH EMGLLDFSYS FLLRAGYLTL YGIEALPRTH ESQAQDRVHS
ADVFHTFRQL DRLLPKLARG SLSVGDKDHM CSVKSRLWKL LSPARLARRA
HRSKWLESYL LHLEEMGVSE EMQARALVLQ LWATQGNMGP AAFWLLLFLL
KNPEALAAVR GELESILWQA EQPVSQTTTL PQKVLDSTPV LDSVLSESLR
LTAAPFITRE VVVDLAMPMA DGREFNLRRG DRLLLFPFLS PQRDPEIYTD
PEVFKYNRFL NPDGSEKKDF YKDGKRLKNY NMPWGAGHNH CLGRSYAVNS
IKQFVFLVLV HLDLELINAD VEIPEFDLSR YGFGLMQPEH DVPVRYRIRP
UCSC uc002xut.4; human.;

Category

Human PTGIS ELISA Kit

Human prostaglandin I2 synthase ELISA Kit;Human CYP8 ELISA Kit;Human CYP8A1 ELISA Kit;Human PGIS ELISA Kit;Human PTGI ELISA Kit;Human prostacyclin synthase ELISA Kit;Human cytochrome P450, family 8, subfamily A, polypeptide 1 ELISA Kit;Human prostaglandin I2 (prostacyclin) synthase ELISA Kit;