Human PSMA1 ELISA Kit

Alternative name
Human 30 kDa prosomal protein ELISA Kit;Human PROS-30 ELISA Kit;Human macropain subunit C2 ELISA Kit;Human multicatalytic endopeptidase complex subunit C2 ELISA Kit;Human proteasome component C2 ELISA Kit;Human proteasome nu chain ELISA Kit;Human HC2 ELISA Kit;Human NU ELISA Kit;Human PROS30 ELISA Kit;Human PSC2 ELISA Kit;Human HEL-S-275 ELISA Kit;Human proteasome subunit alpha 1 ELISA Kit;Human proteasome subunit alpha type-1 ELISA Kit;Human epididymis secretory protein Li 275 ELISA Kit;Human macropain subunit nu ELISA Kit;Human proteasome (prosome, macropain) subunit, alpha type, 1 ELISA Kit;Human proteasome subunit nu ELISA Kit;Human proteasome subunit, alpha-type, 1 ELISA Kit;Human protein P30-33K ELISA Kit;Human testicular tissue protein Li 150 ELISA Kit;
Catalog
E031450
species
Human
GenePSMA1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Proteasome Subunit Alpha Type 1 (PSMa1). No significant cross-reactivity or interference between Proteasome Subunit Alpha Type 1 (PSMa1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.067ng/mL.
Intended UseHuman PSMA1 ELISA Kit allows for the in vitro quantitative determination of PSMA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteasome Subunit Alpha Type 1 (PSMa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteasome Subunit Alpha Type 1 (PSMa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteasome Subunit Alpha Type 1 (PSMa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proteasome Subunit Alpha Type 1 (PSMa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Proteasome subunit alpha type-1 Protein information
Uniprot ID PSA1_HUMAN
Uniprot AC P25786; A8K400; Q53YE8; Q9BRV9;
UniGene Hs.102798;
GeneID 5682
KEGG hsa:5682;
Human Proteasome subunit alpha type-1 Protein SEQUENCE
SEQUENCE 263 AA; 29556 MW; 3F159C5BCEFE8DED CRC64;
MFRNQYDNDV TVWSPQGRIH QIEYAMEAVK QGSATVGLKS KTHAVLVALK
RAQSELAAHQ KKILHVDNHI GISIAGLTAD ARLLCNFMRQ ECLDSRFVFD
RPLPVSRLVS LIGSKTQIPT QRYGRRPYGV GLLIAGYDDM GPHIFQTCPS
ANYFDCRAMS IGARSQSART YLERHMSEFM ECNLNELVKH GLRALRETLP
AEQDLTTKNV SIGIVGKDLE FTIYDDDDVS PFLEGLEERP QRKAQPAQPA
DEPAEKADEP MEH
UCSC uc001mlk.4; human. [P25786-1];

Category

Human PSMA1 ELISA Kit