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Human PDIA2 ELISA Kit

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  • Alternative name

    Human pancreas-specific protein disulfide isomerase ELISA Kit;Human PDIP ELISA Kit;Human PDA2 ELISA Kit;Human PDI ELISA Kit;Human PDIR ELISA Kit;Human protein disulfide isomerase family A member 2 ELISA Kit;Human protein disulfide-isomerase A2 ELISA Kit;Human Rho GDP dissociation inhibitor gamma ELISA Kit;Human pancreatic protein disulfide isomerase ELISA Kit;Human protein disulfide isomerase, pancreatic ELISA Kit;Human protein disulfide isomerase-associated 2 ELISA Kit;
  • Catalog
    E031518
  • species
    Human
  • GenePDIA2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A2 (PDIA2). No significant cross-reactivity or interference between Protein Disulfide Isomerase A2 (PDIA2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.113ng/mL.
  • Intended UseHuman PDIA2 ELISA Kit allows for the in vitro quantitative determination of PDIA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A2 (PDIA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A2 (PDIA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A2 (PDIA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A2 (PDIA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein disulfide-isomerase A2 Protein information
  • Uniprot ID PDIA2_HUMAN
  • Uniprot AC Q13087; A6ZJ64; B4DI27; Q2WGM4; Q4TT67; Q6B010; Q96KJ6; Q9BW95;
  • UniGene Hs.66581;
  • GeneID 64714
  • KEGG hsa:64714;
  • Human Protein disulfide-isomerase A2 Protein SEQUENCE

  • SEQUENCE 525 AA; 58206 MW; B741851AA2C40540 CRC64;

    MSRQLLPVLL LLLLRASCPW GQEQGARSPS EEPPEEEIPK EDGILVLSRH

    TLGLALREHP ALLVEFYAPW CGHCQALAPE YSKAAAVLAA ESMVVTLAKV

    DGPAQRELAE EFGVTEYPTL KFFRNGNRTH PEEYTGPRDA EGIAEWLRRR

    VGPSAMRLED EAAAQALIGG RDLVVIGFFQ DLQDEDVATF LALAQDALDM

    TFGLTDRPRL FQQFGLTKDT VVLFKKFDEG RADFPVDEEL GLDLGDLSRF

    LVTHSMRLVT EFNSQTSAKI FAARILNHLL LFVNQTLAAH RELLAGFGEA

    APRFRGQVLF VVVDVAADNE HVLQYFGLKA EAAPTLRLVN LETTKKYAPV

    DGGPVTAASI TAFCHAVLNG QVKPYLLSQE IPPDWDQRPV KTLVGKNFEQ

    VAFDETKNVF VKFYAPWCTH CKEMAPAWEA LAEKYQDHED IIIAELDATA

    NELDAFAVHG FPTLKYFPAG PGRKVIEYKS TRDLETFSKF LDNGGVLPTE

    EPPEEPAAPF PEPPANSTMG SKEEL

  • UCSC uc002cgo.2; human. [Q13087-1];



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