Human PDIA3 ELISA Kit

Alternative name
Human 58 kDa glucose-regulated protein ELISA Kit;Human 58 kDa microsomal protein ELISA Kit;Human P58 ELISA Kit;Human disulfide isomerase ER-60 ELISA Kit;Human endoplasmic reticulum resident protein 57 ELISA Kit;Human ER protein 57 ELISA Kit;Human ERp57 ELISA Kit;Human endoplasmic reticulum resident protein 60 ELISA Kit;Human ER protein 60 ELISA Kit;Human ERp60 ELISA Kit;Human GRP58 ELISA Kit;Human ER60 ELISA Kit;Human ERp61 ELISA Kit;Human GRP57 ELISA Kit;Human HEL-S-269 ELISA Kit;Human HEL-S-93n ELISA Kit;Human HsT17083 ELISA Kit;Human PI-PLC ELISA Kit;Human protein disulfide isomerase family A member 3 ELISA Kit;Human protein disulfide-isomerase A3 ELISA Kit;Human endoplasmic reticulum P58 ELISA Kit;Human epididymis secretory protein Li 269 ELISA Kit;Human epididymis secretory sperm binding protein Li 93n ELISA Kit;Human glucose regulated protein, 58kDa ELISA Kit;Human phospholipase C-alpha ELISA Kit;Human protein disulfide isomerase-associated 3 ELISA Kit;
Catalog
E031522
species
Human
GenePDIA3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A3 (PDIA3). No significant cross-reactivity or interference between Protein Disulfide Isomerase A3 (PDIA3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.061ng/mL.
Intended UseHuman PDIA3 ELISA Kit allows for the in vitro quantitative determination of PDIA3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A3 (PDIA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A3 (PDIA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A3 (PDIA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A3 (PDIA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Protein disulfide-isomerase A3 Protein information
Uniprot ID PDIA3_HUMAN
Uniprot AC P30101; Q13453; Q14255; Q8IYF8; Q9UMU7;
UniGene Hs.591095;
GeneID 2923
KEGG hsa:2923;
Human Protein disulfide-isomerase A3 Protein SEQUENCE
SEQUENCE 505 AA; 56782 MW; 529E5B6692D0D7E9 CRC64;
MRLRRLALFP GVALLLAAAR LAAASDVLEL TDDNFESRIS DTGSAGLMLV
EFFAPWCGHC KRLAPEYEAA ATRLKGIVPL AKVDCTANTN TCNKYGVSGY
PTLKIFRDGE EAGAYDGPRT ADGIVSHLKK QAGPASVPLR TEEEFKKFIS
DKDASIVGFF DDSFSEAHSE FLKAASNLRD NYRFAHTNVE SLVNEYDDNG
EGIILFRPSH LTNKFEDKTV AYTEQKMTSG KIKKFIQENI FGICPHMTED
NKDLIQGKDL LIAYYDVDYE KNAKGSNYWR NRVMMVAKKF LDAGHKLNFA
VASRKTFSHE LSDFGLESTA GEIPVVAIRT AKGEKFVMQE EFSRDGKALE
RFLQDYFDGN LKRYLKSEPI PESNDGPVKV VVAENFDEIV NNENKDVLIE
FYAPWCGHCK NLEPKYKELG EKLSKDPNIV IAKMDATAND VPSPYEVRGF
PTIYFSPANK KLNPKKYEGG RELSDFISYL QREATNPPVI QEEKPKKKKK
AQEDL

Category

Human PDIA3 ELISA Kit