Human FEM1A ELISA Kit

Alternative name
Human FEM1a ELISA Kit;Human FEM1-alpha ELISA Kit;Human prostaglandin E receptor 4-associated protein ELISA Kit;Human EPRAP ELISA Kit;Human fem-1 homolog A ELISA Kit;Human protein fem-1 homolog A ELISA Kit;
Catalog
E031551
species
Human
GeneFEM1A
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseHuman FEM1A ELISA Kit allows for the in vitro quantitative determination of FEM1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human FEM1A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human FEM1A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FEM1A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman FEM1A can be calculated.
Human Protein fem-1 homolog A Protein information
Uniprot ID FEM1A_HUMAN
Uniprot AC Q9BSK4; B2RDI3; Q711P8; Q9NPN7; Q9NPW8;
UniGene Hs.515082;
GeneID 55527
KEGG hsa:55527;
Human Protein fem-1 homolog A Protein SEQUENCE
SEQUENCE 669 AA; 73639 MW; FF0010DE5207D48F CRC64;
MDLRTAVYNA ARDGKLQLLQ KLLSGRSREE LDELTGEVAG GGTPLLIAAR
YGHLDVVEYL VDRCGASVEA GGSVHFDGET IEGAPPLWAA SAAGHLDVVR
SLLRRGASVN RTTRTNSTPL RAACFDGHLE VVRYLVGEHQ ADLEVANRHG
HTCLMISCYK GHREIARYLL EQGAQVNRRS AKGNTALHDC AESGSLEILQ
LLLGCKARME RDGYGMTPLL AASVTGHTNI VEYLIQEQPG QEQVAGGEAQ
PGLPQEDPST SQGCAQPQGA PCCSSSPEEP LNGESYESCC PTSREAAVEA
LELLGATYVD KKRDLLGALK HWRRAMELRH QGGEYLPKPE PPQLVLAYDY
SREVNTTEEL EALITDPDEM RMQALLIRER ILGPSHPDTS YYIRYRGAVY
ADSGNFERCI RLWKYALDMQ QSNLEPLSPM TASSFLSFAE LFSYVLQDRA
AKGSLGTQIG FADLMGVLTK GVREVERALQ LPREPGDSAQ FTKALAIILH
LLYLLEKVEC TPSQEHLKHQ TVYRLLKCAP RGKNGFTPLH MAVDKDTTNV
GRYPVGRFPS LHVVKVLLDC GADPDSRDFD NNTPLHIAAQ NNCPAIMNAL
IEAGAHMDAT NAFKKTAYEL LDEKLLARGT MQPFNYVTLQ CLAARALDKN
KIPYKGFIPE DLEAFIELH
UCSC uc002mbf.4; human.;

Category

Human FEM1A ELISA Kit

Human FEM1a ELISA Kit;Human FEM1-alpha ELISA Kit;Human prostaglandin E receptor 4-associated protein ELISA Kit;Human EPRAP ELISA Kit;Human fem-1 homolog A ELISA Kit;Human protein fem-1 homolog A ELISA Kit;