Human PIAS3 ELISA Kit

Alternative name
Human protein inhibitor of activated STAT protein 3 ELISA Kit;Human ZMIZ5 ELISA Kit;Human protein inhibitor of activated STAT 3 ELISA Kit;Human E3 SUMO-protein ligase PIAS3 ELISA Kit;Human zinc finger, MIZ-type containing 5 ELISA Kit;
Catalog
E031556
species
Human
GenePIAS3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Inhibitor Of Activated STAT 3 (PIAS3). No significant cross-reactivity or interference between Protein Inhibitor Of Activated STAT 3 (PIAS3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.064ng/mL.
Intended UseHuman PIAS3 ELISA Kit allows for the in vitro quantitative determination of PIAS3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Inhibitor Of Activated STAT 3 (PIAS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Inhibitor Of Activated STAT 3 (PIAS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Inhibitor Of Activated STAT 3 (PIAS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Inhibitor Of Activated STAT 3 (PIAS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human E3 SUMO-protein ligase PIAS3 Protein information
Uniprot ID PIAS3_HUMAN
Uniprot AC Q9Y6X2; Q9UFI3;
UniGene Hs.435761;
GeneID 10401
KEGG hsa:10401;
Human E3 SUMO-protein ligase PIAS3 Protein SEQUENCE
SEQUENCE 628 AA; 68017 MW; 7C06EF599D48F87D CRC64;
MAELGELKHM VMSFRVSELQ VLLGFAGRNK SGRKHELLAK ALHLLKSSCA
PSVQMKIKEL YRRRFPRKTL GPSDLSLLSL PPGTSPVGSP GPLAPIPPTL
LAPGTLLGPK REVDMHPPLP QPVHPDVTMK PLPFYEVYGE LIRPTTLAST
SSQRFEEAHF TFALTPQQVQ QILTSREVLP GAKCDYTIQV QLRFCLCETS
CPQEDYFPPN LFVKVNGKLC PLPGYLPPTK NGAEPKRPSR PINITPLARL
SATVPNTIVV NWSSEFGRNY SLSVYLVRQL TAGTLLQKLR AKGIRNPDHS
RALIKEKLTA DPDSEVATTS LRVSLMCPLG KMRLTVPCRA LTCAHLQSFD
AALYLQMNEK KPTWTCPVCD KKAPYESLII DGLFMEILSS CSDCDEIQFM
EDGSWCPMKP KKEASEVCPP PGYGLDGLQY SPVQGGDPSE NKKKVEVIDL
TIESSSDEED LPPTKKHCSV TSAAIPALPG SKGVLTSGHQ PSSVLRSPAM
GTLGGDFLSS LPLHEYPPAF PLGADIQGLD LFSFLQTESQ HYGPSVITSL
DEQDALGHFF QYRGTPSHFL GPLAPTLGSS HCSATPAPPP GRVSSIVAPG
GALREGHGGP LPSGPSLTGC RSDIISLD
UCSC uc001eoc.2; human.;

Category

Human PIAS3 ELISA Kit

Human protein inhibitor of activated STAT protein 3 ELISA Kit;Human ZMIZ5 ELISA Kit;Human protein inhibitor of activated STAT 3 ELISA Kit;Human E3 SUMO-protein ligase PIAS3 ELISA Kit;Human zinc finger, MIZ-type containing 5 ELISA Kit;