Human PRKCA ELISA Kit

Alternative name
Human PKC-A ELISA Kit;Human PKC-alpha ELISA Kit;Human PKCA ELISA Kit;Human PRKACA ELISA Kit;Human AAG6 ELISA Kit;Human protein kinase C alpha ELISA Kit;Human protein kinase C alpha type ELISA Kit;Human aging-associated gene 6 ELISA Kit;
Catalog
E031586
species
Human
GenePRKCA
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman PRKCA ELISA Kit allows for the in vitro quantitative determination of PRKCA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PKCalpha antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PKCalpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PKCalpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PKCalpha can be calculated.
Human Protein kinase C alpha type Protein information
Uniprot ID KPCA_HUMAN
Uniprot AC P17252; B5BU22; Q15137; Q32M72; Q96RE4;
UniGene Hs.531704; Hs.708867;
GeneID 5578
KEGG hsa:5578;
Human Protein kinase C alpha type Protein SEQUENCE
SEQUENCE 672 AA; 76750 MW; 9EB157789A062349 CRC64;
MADVFPGNDS TASQDVANRF ARKGALRQKN VHEVKDHKFI ARFFKQPTFC
SHCTDFIWGF GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPDTDDPRS
KHKFKIHTYG SPTFCDHCGS LLYGLIHQGM KCDTCDMNVH KQCVINVPSL
CGMDHTEKRG RIYLKAEVAD EKLHVTVRDA KNLIPMDPNG LSDPYVKLKL
IPDPKNESKQ KTKTIRSTLN PQWNESFTFK LKPSDKDRRL SVEIWDWDRT
TRNDFMGSLS FGVSELMKMP ASGWYKLLNQ EEGEYYNVPI PEGDEEGNME
LRQKFEKAKL GPAGNKVISP SEDRKQPSNN LDRVKLTDFN FLMVLGKGSF
GKVMLADRKG TEELYAIKIL KKDVVIQDDD VECTMVEKRV LALLDKPPFL
TQLHSCFQTV DRLYFVMEYV NGGDLMYHIQ QVGKFKEPQA VFYAAEISIG
LFFLHKRGII YRDLKLDNVM LDSEGHIKIA DFGMCKEHMM DGVTTRTFCG
TPDYIAPEII AYQPYGKSVD WWAYGVLLYE MLAGQPPFDG EDEDELFQSI
MEHNVSYPKS LSKEAVSVCK GLMTKHPAKR LGCGPEGERD VREHAFFRRI
DWEKLENREI QPPFKPKVCG KGAENFDKFF TRGQPVLTPP DQLVIANIDQ
SDFEGFSYVN PQFVHPILQS AV
UCSC uc002jfp.2; human.;

Category

Human PRKCA ELISA Kit

Human PKC-A ELISA Kit;Human PKC-alpha ELISA Kit;Human PKCA ELISA Kit;Human PRKACA ELISA Kit;Human AAG6 ELISA Kit;Human protein kinase C alpha ELISA Kit;Human protein kinase C alpha type ELISA Kit;Human aging-associated gene 6 ELISA Kit;