Human PRKCE ELISA Kit

Alternative name
Human nPKC-epsilon ELISA Kit;Human PKCE ELISA Kit;Human protein kinase C epsilon ELISA Kit;Human protein kinase C epsilon type ELISA Kit;
Catalog
E031601
species
Human
GenePRKCE
SpecificityThis assay has high sensitivity and excellent specificity for detection of PRKCE . No significant cross-reactivity or interference between PRKCE and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 0.938ng/ml
Intended UseHuman PRKCE ELISA Kit allows for the in vitro quantitative determination of PRKCE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PRKCE antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PRKCE antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PRKCE amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PRKCE can be calculated.
Human Protein kinase C epsilon type Protein information
Uniprot ID KPCE_HUMAN
Uniprot AC Q02156; B0LPH7; Q32MQ3; Q53SL4; Q53SM5; Q9UE81;
UniGene Hs.580351; Hs.677120;
GeneID 5581
KEGG hsa:5581;
Human Protein kinase C epsilon type Protein SEQUENCE
SEQUENCE 737 AA; 83674 MW; 85032D0A091A1F7F CRC64;
MVVFNGLLKI KICEAVSLKP TAWSLRHAVG PRPQTFLLDP YIALNVDDSR
IGQTATKQKT NSPAWHDEFV TDVCNGRKIE LAVFHDAPIG YDDFVANCTI
QFEELLQNGS RHFEDWIDLE PEGRVYVIID LSGSSGEAPK DNEERVFRER
MRPRKRQGAV RRRVHQVNGH KFMATYLRQP TYCSHCRDFI WGVIGKQGYQ
CQVCTCVVHK RCHELIITKC AGLKKQETPD QVGSQRFSVN MPHKFGIHNY
KVPTFCDHCG SLLWGLLRQG LQCKVCKMNV HRRCETNVAP NCGVDARGIA
KVLADLGVTP DKITNSGQRR KKLIAGAESP QPASGSSPSE EDRSKSAPTS
PCDQEIKELE NNIRKALSFD NRGEEHRAAS SPDGQLMSPG ENGEVRQGQA
KRLGLDEFNF IKVLGKGSFG KVMLAELKGK DEVYAVKVLK KDVILQDDDV
DCTMTEKRIL ALARKHPYLT QLYCCFQTKD RLFFVMEYVN GGDLMFQIQR
SRKFDEPRSR FYAAEVTSAL MFLHQHGVIY RDLKLDNILL DAEGHCKLAD
FGMCKEGILN GVTTTTFCGT PDYIAPEILQ ELEYGPSVDW WALGVLMYEM
MAGQPPFEAD NEDDLFESIL HDDVLYPVWL SKEAVSILKA FMTKNPHKRL
GCVASQNGED AIKQHPFFKE IDWVLLEQKK IKPPFKPRIK TKRDVNNFDQ
DFTREEPVLT LVDEAIVKQI NQEEFKGFSY FGEDLMP
UCSC uc002rut.4; human.;

Category

Human PRKCE ELISA Kit

Human nPKC-epsilon ELISA Kit;Human PKCE ELISA Kit;Human protein kinase C epsilon ELISA Kit;Human protein kinase C epsilon type ELISA Kit;