Human PKN1 ELISA Kit

Alternative name
Human protease-activated kinase 1 ELISA Kit;Human PAK-1 ELISA Kit;Human protein kinase C-like 1 ELISA Kit;Human protein kinase C-like PKN ELISA Kit;Human protein kinase PKN-alpha ELISA Kit;Human Protein-kinase C-related kinase 1 ELISA Kit;Human Serine-threonine protein kinase N ELISA Kit;Human PAK1 ELISA Kit;Human PKN ELISA Kit;Human PRK1 ELISA Kit;Human PRKCL1 ELISA Kit;Human DBK ELISA Kit;Human PKN-ALPHA ELISA Kit;Human protein kinase N1 ELISA Kit;Human serine/threonine-protein kinase N1 ELISA Kit;Human protein kinase C-related kinase 1 ELISA Kit;Human serine-threonine kinase N ELISA Kit;Human serine/threonine protein kinase N ELISA Kit;
Catalog
E031644
species
Human
GenePKN1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Kinase N1 (PKN1). No significant cross-reactivity or interference between Protein Kinase N1 (PKN1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.117ng/mL.
Intended UseHuman PKN1 ELISA Kit allows for the in vitro quantitative determination of PKN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase N1 (PKN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase N1 (PKN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase N1 (PKN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase N1 (PKN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Serine/threonine-protein kinase N1 Protein information
Uniprot ID PKN1_HUMAN
Uniprot AC Q16512; A8K7W5; B2R9R4; B3KVN3; Q15143; Q504U4; Q8IUV5; Q9UD44;
UniGene Hs.466044;
GeneID 5585
KEGG hsa:5585;
Human Serine/threonine-protein kinase N1 Protein SEQUENCE
SEQUENCE 942 AA; 103932 MW; 61360295EC70BB8E CRC64;
MASDAVQSEP RSWSLLEQLG LAGADLAAPG VQQQLELERE RLRREIRKEL
KLKEGAENLR RATTDLGRSL GPVELLLRGS SRRLDLLHQQ LQELHAHVVL
PDPAATHDGP QSPGAGGPTC SATNLSRVAG LEKQLAIELK VKQGAENMIQ
TYSNGSTKDR KLLLTAQQML QDSKTKIDII RMQLRRALQA GQLENQAAPD
DTQGSPDLGA VELRIEELRH HFRVEHAVAE GAKNVLRLLS AAKAPDRKAV
SEAQEKLTES NQKLGLLREA LERRLGELPA DHPKGRLLRE ELAAASSAAF
STRLAGPFPA THYSTLCKPA PLTGTLEVRV VGCRDLPETI PWNPTPSMGG
PGTPDSRPPF LSRPARGLYS RSGSLSGRSS LKAEAENTSE VSTVLKLDNT
VVGQTSWKPC GPNAWDQSFT LELERARELE LAVFWRDQRG LCALKFLKLE
DFLDNERHEV QLDMEPQGCL VAEVTFRNPV IERIPRLRRQ KKIFSKQQGK
AFQRARQMNI DVATWVRLLR RLIPNATGTG TFSPGASPGS EARTTGDISV
EKLNLGTDSD SSPQKSSRDP PSSPSSLSSP IQESTAPELP SETQETPGPA
LCSPLRKSPL TLEDFKFLAV LGRGHFGKVL LSEFRPSGEL FAIKALKKGD
IVARDEVESL MCEKRILAAV TSAGHPFLVN LFGCFQTPEH VCFVMEYSAG
GDLMLHIHSD VFSEPRAIFY SACVVLGLQF LHEHKIVYRD LKLDNLLLDT
EGYVKIADFG LCKEGMGYGD RTSTFCGTPE FLAPEVLTDT SYTRAVDWWG
LGVLLYEMLV GESPFPGDDE EEVFDSIVND EVRYPRFLSA EAIGIMRRLL
RRNPERRLGS SERDAEDVKK QPFFRTLGWE ALLARRLPPP FVPTLSGRTD
VSNFDEEFTG EAPTLSPPRD ARPLTAAEQA AFLDFDFVAG GC
UCSC uc002myp.4; human. [Q16512-1];

Category

Human PKN1 ELISA Kit