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Human PPP1CA ELISA Kit

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  • Alternative name

    Human PP-1A ELISA Kit;Human PPP1A ELISA Kit;Human PP1A ELISA Kit;Human PP1alpha ELISA Kit;Human protein phosphatase 1 catalytic subunit alpha ELISA Kit;Human serine/threonine-protein phosphatase PP1-alpha catalytic subunit ELISA Kit;Human protein phosphatase 1, catalytic subunit, alpha isoform ELISA Kit;Human protein phosphatase 1, catalytic subunit, alpha isozyme ELISA Kit;Human serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit ELISA Kit;Human testicular tissue protein Li 155 ELISA Kit;
  • Catalog
    E031704
  • species
    Human
  • GenePPP1CA
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human PPP1CA. No significant cross-reactivity or interference between human PPP1CA and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human PPP1CA is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
  • Intended UseHuman PPP1CA ELISA Kit allows for the in vitro quantitative determination of PPP1CA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PPP1CA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPP1CA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP1CA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP1CA bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Protein information
  • Uniprot ID PP1A_HUMAN
  • Uniprot AC P62136; A6NNR3; B2R908; P08129; P20653; P22802; Q07161;
  • UniGene Hs.183994;
  • GeneID 5499
  • KEGG hsa:5499;
  • Human Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Protein SEQUENCE

  • SEQUENCE 330 AA; 37512 MW; 60C37E1AD9831DAC CRC64;

    MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP

    ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS

    LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK

    TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL

    LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV

    VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD

    KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK

  • UCSC uc001oku.2; human. [P62136-1];



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