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Human PPP1R1B ELISA Kit

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  • Alternative name

    Human DARPP-32 ELISA Kit;Human Dopamine- and cAMP-regulated neuronal phosphoprotein ELISA Kit;Human DARPP32 ELISA Kit;Human protein phosphatase 1 regulatory inhibitor subunit 1B ELISA Kit;Human protein phosphatase 1 regulatory subunit 1B ELISA Kit;Human dopamine and cAMP-regulated neuronal phosphoprotein 32 ELISA Kit;
  • Catalog
    E031736
  • species
    Human
  • GenePPP1R1B
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Phosphatase 1, Regulatory Subunit 1B (PPP1R1B). No significant cross-reactivity or interference between Protein Phosphatase 1, Regulatory Subunit 1B (PPP1R1B) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.057ng/mL.
  • Intended UseHuman PPP1R1B ELISA Kit allows for the in vitro quantitative determination of PPP1R1B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Phosphatase 1, Regulatory Subunit 1B (PPP1R1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Phosphatase 1, Regulatory Subunit 1B (PPP1R1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Phosphatase 1, Regulatory Subunit 1B (PPP1R1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Phosphatase 1, Regulatory Subunit 1B (PPP1R1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein phosphatase 1 regulatory subunit 1B Protein information
  • Uniprot ID PPR1B_HUMAN
  • Uniprot AC Q9UD71; Q547V9; Q547W0; Q9H7G1;
  • UniGene Hs.286192;
  • GeneID 84152
  • KEGG hsa:84152;
  • Human Protein phosphatase 1 regulatory subunit 1B Protein SEQUENCE

  • SEQUENCE 204 AA; 22963 MW; E1CE7DDD29D2D4FB CRC64;

    MDPKDRKKIQ FSVPAPPSQL DPRQVEMIRR RRPTPAMLFR LSEHSSPEEE

    ASPHQRASGE GHHLKSKRPN PCAYTPPSLK AVQRIAESHL QSISNLNENQ

    ASEEEDELGE LRELGYPREE DEEEEEDDEE EEEEEDSQAE VLKVIRQSAG

    QKTTCGQGLE GPWERPPPLD ESERDGGSED QVEDPALSEP GEEPQRPSPS

    EPGT

  • UCSC uc002hrz.4; human. [Q9UD71-1];



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