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Alternative name
Serine/threonine-protein phosphatase with EF-hands 1 ELISA KIT; Protein phosphatase with EF calcium-binding domain ELISA KIT; PPEF ELISA KIT; Serine/threonine-protein phosphatase 7 ELISA KIT; PP7PPEF1 ELISA KIT; PPEF ELISA KIT; PPP7C ELISA KIT; PPEF-1 ELISA KIT; PPEF ELISA KIT; PP7 ELISA KIT
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Catalog
E031762
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species
Human
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GenePPEF1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human PPEF1. No significant cross-reactivity or interference between human PPEF1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human PPEF1 is typically less than 9.76 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
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Intended UseHuman PPEF1 ELISA Kit allows for the in vitro quantitative determination of PPEF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PPEF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPEF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPEF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPEF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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