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Human PTPRN ELISA Kit

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  • Alternative name

    Human R-PTP-N ELISA Kit;Human islet cell antigen 512 ELISA Kit;Human ICA 512 ELISA Kit;Human islet cell autoantigen 3 ELISA Kit;Human PTP IA-2 ELISA Kit;Human ICA512-NTF ELISA Kit;Human ICA512-TMF ELISA Kit;Human ICA512-CCF ELISA Kit;Human ICA3 ELISA Kit;Human ICA512 ELISA Kit;Human IA-2 ELISA Kit;Human IA-2/PTP ELISA Kit;Human IA2 ELISA Kit;Human protein tyrosine phosphatase, receptor type N ELISA Kit;Human receptor-type tyrosine-protein phosphatase-like N ELISA Kit;Human islet cell antigen 2 ELISA Kit;Human protein tyrosine phosphatase-like N ELISA Kit;
  • Catalog
    E031855
  • species
    Human
  • GenePTPRN
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PTPRN. No significant cross-reactivity or interference between PTPRN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTPRN and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PTPRN ELISA Kit allows for the in vitro quantitative determination of PTPRN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Intended Uses: This PTPRN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PTPRN. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||PTPRN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTPRN antibody and an PTPRN-HRP conjugate. The assay sample and buffer are incubated together with PTPRN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTPRN concentration since PTPRN from samples and PTPRN-HRP conjugate compete for the anti-PTPRN antibody binding site. Since the number of sites is limited, as more sites are occupied by PTPRN from the sample, fewer sites are left to bind PTPRN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTPRN concentration in each sample is interpolated from this standard curve.
  • Human ICA512-cleaved cytosolic fragment Protein information
  • Uniprot ID PTPRN_HUMAN
  • Uniprot AC Q16849; B4DK12; F5GZS3; Q08319; Q53QD6; Q6NSL1;
  • UniGene Hs.89655;
  • GeneID 5798
  • KEGG hsa:5798;
  • Human ICA512-cleaved cytosolic fragment Protein SEQUENCE

  • SEQUENCE 979 AA; 105848 MW; A852B9063D29399D CRC64;

    MRRPRRPGGL GGSGGLRLLL CLLLLSSRPG GCSAVSAHGC LFDRRLCSHL

    EVCIQDGLFG QCQVGVGQAR PLLQVTSPVL QRLQGVLRQL MSQGLSWHDD

    LTQYVISQEM ERIPRLRPPE PRPRDRSGLA PKRPGPAGEL LLQDIPTGSA

    PAAQHRLPQP PVGKGGAGAS SSLSPLQAEL LPPLLEHLLL PPQPPHPSLS

    YEPALLQPYL FHQFGSRDGS RVSEGSPGMV SVGPLPKAEA PALFSRTASK

    GIFGDHPGHS YGDLPGPSPA QLFQDSGLLY LAQELPAPSR ARVPRLPEQG

    SSSRAEDSPE GYEKEGLGDR GEKPASPAVQ PDAALQRLAA VLAGYGVELR

    QLTPEQLSTL LTLLQLLPKG AGRNPGGVVN VGADIKKTME GPVEGRDTAE

    LPARTSPMPG HPTASPTSSE VQQVPSPVSS EPPKAARPPV TPVLLEKKSP

    LGQSQPTVAG QPSARPAAEE YGYIVTDQKP LSLAAGVKLL EILAEHVHMS

    SGSFINISVV GPALTFRIRH NEQNLSLADV TQQAGLVKSE LEAQTGLQIL

    QTGVGQREEA AAVLPQTAHS TSPMRSVLLT LVALAGVAGL LVALAVALCV

    RQHARQQDKE RLAALGPEGA HGDTTFEYQD LCRQHMATKS LFNRAEGPPE

    PSRVSSVSSQ FSDAAQASPS SHSSTPSWCE EPAQANMDIS TGHMILAYME

    DHLRNRDRLA KEWQALCAYQ AEPNTCATAQ GEGNIKKNRH PDFLPYDHAR

    IKLKVESSPS RSDYINASPI IEHDPRMPAY IATQGPLSHT IADFWQMVWE

    SGCTVIVMLT PLVEDGVKQC DRYWPDEGAS LYHVYEVNLV SEHIWCEDFL

    VRSFYLKNVQ TQETRTLTQF HFLSWPAEGT PASTRPLLDF RRKVNKCYRG

    RSCPIIVHCS DGAGRTGTYI LIDMVLNRMA KGVKEIDIAA TLEHVRDQRP

    GLVRSKDQFE FALTAVAEEV NAILKALPQ

  • UCSC uc002vkz.3; human. [Q16849-1];



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