Human PTPN1 ELISA Kit

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  • Alternative name

    Human protein-tyrosine phosphatase 1B ELISA Kit;Human PTP-1B ELISA Kit;Human PTP1B ELISA Kit;Human protein tyrosine phosphatase, non-receptor type 1 ELISA Kit;Human tyrosine-protein phosphatase non-receptor type 1 ELISA Kit;Human protein tyrosine phosphatase, placental ELISA Kit;

  • Catalog
    E031872
  • species
    Human
  • GenePTPN1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PTPN1. No significant cross-reactivity or interference between PTPN1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of PTPN1 is typically less than 0.054ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding tw
  • Intended UseHuman PTPN1 ELISA Kit allows for the in vitro quantitative determination of PTPN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTPN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PTPN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PTPN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PTPN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Tyrosine-protein phosphatase non-receptor type 1 Protein information
  • Uniprot ID PTN1_HUMAN
  • Uniprot AC P18031; Q5TGD8; Q9BQV9; Q9NQQ4;
  • UniGene Hs.417549;
  • GeneID 5770
  • KEGG hsa:5770;
  • Human Tyrosine-protein phosphatase non-receptor type 1 Protein SEQUENCE
  • SEQUENCE 435 AA; 49967 MW; 802377DCD33F41FD CRC64;

    MEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS

    PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW

    EQKSRGVVML NRVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK

    SYYTVRQLEL ENLTTQETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE

    SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL

    LEMRKFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE

    PPPEHIPPPP RPPKRILEPH NGKCREFFPN HQWVKEETQE DKDCPIKEEK

    GSPLNAAPYG IESMSQDTEV RSRVVGGSLR GAQAASPAKG EPSLPEKDED

    HALSYWKPFL VNMCVATVLT AGAYLCYRFL FNSNT

  • UCSC uc002xvl.5; human.;



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