Human PTPN4 ELISA Kit

Alternative name
Human Protein-tyrosine phosphatase MEG1 ELISA Kit;Human MEG ELISA Kit;Human PTPase-MEG1 ELISA Kit;Human PTPMEG ELISA Kit;Human PTPMEG1 ELISA Kit;Human protein tyrosine phosphatase, non-receptor type 4 ELISA Kit;Human tyrosine-protein phosphatase non-receptor type 4 ELISA Kit;Human megakaryocyte phosphatase ELISA Kit;Human megakaryocyte protein-tyrosine phosphatase ELISA Kit;Human protein tyrosine phosphatase MEG1 ELISA Kit;Human protein tyrosine phosphatase, non-receptor type 4 (megakaryocyte) ELISA Kit;
Catalog
E031874
species
Human
GenePTPN4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman PTPN4 ELISA Kit allows for the in vitro quantitative determination of PTPN4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalFor Samples: Cell culture fluid & body fluid & tissue homogenate serum or blood plasma Intended Uses: This PICP ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of PICP in the sample, this PICP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus PICP concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of PICP utilizes a polyclonal anti-PICP antibody and an PICP-HRP conjugate. The assay sample and buffer are incubated together with PICP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PICP concentration since PICP from samples and PICP-HRP conjugate compete for the anti-PICP antibody binding site. Since the number of sites is limited, as more sites are occupied by PICP from the sample, fewer sites are left to bind PICP-HRP conjugate. Standards of known PICP concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (O.D.) to the concentration of PICP. The PICP concentration in each sample is interpolated from this standard curve.
Human Tyrosine-protein phosphatase non-receptor type 4 Protein information
Uniprot ID PTN4_HUMAN
Uniprot AC P29074; B2RBV8; Q9UDA7;
UniGene Hs.469809;
GeneID 5775
KEGG hsa:5775;
Human Tyrosine-protein phosphatase non-receptor type 4 Protein SEQUENCE
SEQUENCE 926 AA; 105911 MW; 4DAC6A87A675CFB0 CRC64;
MTSRFRLPAG RTYNVRASEL ARDRQHTEVV CNILLLDNTV QAFKVNKHDQ
GQVLLDVVFK HLDLTEQDYF GLQLADDSTD NPRWLDPNKP IRKQLKRGSP
YSLNFRVKFF VSDPNKLQEE YTRYQYFLQI KQDILTGRLP CPSNTAALLA
SFAVQSELGD YDQSENLSGY LSDYSFIPNQ PQDFEKEIAK LHQQHIGLSP
AEAEFNYLNT ARTLELYGVE FHYARDQSNN EIMIGVMSGG ILIYKNRVRM
NTFPWLKIVK ISFKCKQFFI QLRKELHESR ETLLGFNMVN YRACKNLWKA
CVEHHTFFRL DRPLPPQKNF FAHYFTLGSK FRYCGRTEVQ SVQYGKEKAN
KDRVFARSPS KPLARKLMDW EVVSRNSISD DRLETQSLPS RSPPGTPNHR
NSTFTQEGTR LRPSSVGHLV DHMVHTSPSE VFVNQRSPSS TQANSIVLES
SPSQETPGDG KPPALPPKQS KKNSWNQIHY SHSQQDLESH INETFDIPSS
PEKPTPNGGI PHDNLVLIRM KPDENGRFGF NVKGGYDQKM PVIVSRVAPG
TPADLCVPRL NEGDQVVLIN GRDIAEHTHD QVVLFIKASC ERHSGELMLL
VRPNAVYDVV EEKLENEPDF QYIPEKAPLD SVHQDDHSLR ESMIQLAEGL
ITGTVLTQFD QLYRKKPGMT MSCAKLPQNI SKNRYRDISP YDATRVILKG
NEDYINANYI NMEIPSSSII NQYIACQGPL PHTCTDFWQM TWEQGSSMVV
MLTTQVERGR VKCHQYWPEP TGSSSYGCYQ VTCHSEEGNT AYIFRKMTLF
NQEKNESRPL TQIQYIAWPD HGVPDDSSDF LDFVCHVRNK RAGKEEPVVV
HCSAGIGRTG VLITMETAMC LIECNQPVYP LDIVRTMRDQ RAMMIQTPSQ
YRFVCEAILK VYEEGFVKPL TTSTNK
UCSC uc002tmf.3; human.;

Category

Human PTPN4 ELISA Kit