Human WNT2 ELISA Kit

Alternative name
Human int-1-like protein 1 ELISA Kit;Human Int-1-related protein ELISA Kit;Human IRP ELISA Kit;Human INT1L1 ELISA Kit;Human Wnt family member 2 ELISA Kit;Human protein Wnt-2 ELISA Kit;Human secreted growth factor ELISA Kit;Human wingless-type MMTV integration site family member 2 ELISA Kit;
Catalog
E031918
species
Human
GeneWNT2
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman WNT2 ELISA Kit allows for the in vitro quantitative determination of WNT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human WNT2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human WNT2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human WNT2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human WNT2 can be calculated.
Human Protein Wnt-2 Protein information
Uniprot ID WNT2_HUMAN
Uniprot AC P09544; A4D0V1; Q75N05; Q9UDP9;
UniGene Hs.567356;
GeneID 7472
KEGG hsa:7472;
Human Protein Wnt-2 Protein SEQUENCE
SEQUENCE 360 AA; 40418 MW; AA96325BF68C4788 CRC64;
MNAPLGGIWL WLPLLLTWLT PEVNSSWWYM RATGGSSRVM CDNVPGLVSS
QRQLCHRHPD VMRAISQGVA EWTAECQHQF RQHRWNCNTL DRDHSLFGRV
LLRSSRESAF VYAISSAGVV FAITRACSQG EVKSCSCDPK KMGSAKDSKG
IFDWGGCSDN IDYGIKFARA FVDAKERKGK DARALMNLHN NRAGRKAVKR
FLKQECKCHG VSGSCTLRTC WLAMADFRKT GDYLWRKYNG AIQVVMNQDG
TGFTVANERF KKPTKNDLVY FENSPDYCIR DREAGSLGTA GRVCNLTSRG
MDSCEVMCCG RGYDTSHVTR MTKCGCKFHW CCAVRCQDCL EALDVHTCKA
PKNADWTTAT
UCSC uc003viz.4; human.;

Category

Human WNT2 ELISA Kit

Human int-1-like protein 1 ELISA Kit;Human Int-1-related protein ELISA Kit;Human IRP ELISA Kit;Human INT1L1 ELISA Kit;Human Wnt family member 2 ELISA Kit;Human protein Wnt-2 ELISA Kit;Human secreted growth factor ELISA Kit;Human wingless-type MMTV integration site family member 2 ELISA Kit;