Human F2R ELISA Kit

Alternative name
Human PAR-1 ELISA Kit;Human Coagulation factor II receptor ELISA Kit;Human Thrombin receptor ELISA Kit;Human CF2R ELISA Kit;Human PAR1 ELISA Kit;Human TR ELISA Kit;Human HTR ELISA Kit;Human coagulation factor II thrombin receptor ELISA Kit;Human proteinase-activated receptor 1 ELISA Kit;Human protease-activated receptor 1 ELISA Kit;
Catalog
E032020
species
Human
GeneF2R
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman F2R ELISA Kit allows for the in vitro quantitative determination of F2R , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human PAR1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human PAR1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PAR1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman PAR1can be calculated.
Human Proteinase-activated receptor 1 Protein information
Uniprot ID PAR1_HUMAN
Uniprot AC P25116; Q53XV0; Q96RF7; Q9BUN4;
UniGene Hs.482562;
GeneID 2149
KEGG hsa:2149;
Human Proteinase-activated receptor 1 Protein SEQUENCE
SEQUENCE 425 AA; 47441 MW; 41B742A99EEC96AB CRC64;
MGPRRLLLVA ACFSLCGPLL SARTRARRPE SKATNATLDP RSFLLRNPND
KYEPFWEDEE KNESGLTEYR LVSINKSSPL QKQLPAFISE DASGYLTSSW
LTLFVPSVYT GVFVVSLPLN IMAIVVFILK MKVKKPAVVY MLHLATADVL
FVSVLPFKIS YYFSGSDWQF GSELCRFVTA AFYCNMYASI LLMTVISIDR
FLAVVYPMQS LSWRTLGRAS FTCLAIWALA IAGVVPLLLK EQTIQVPGLN
ITTCHDVLNE TLLEGYYAYY FSAFSAVFFF VPLIISTVCY VSIIRCLSSS
AVANRSKKSR ALFLSAAVFC IFIICFGPTN VLLIAHYSFL SHTSTTEAAY
FAYLLCVCVS SISCCIDPLI YYYASSECQR YVYSILCCKE SSDPSSYNSS
GQLMASKMDT CSSNLNNSIY KKLLT
UCSC uc003ken.5; human.;

Category

Human F2R ELISA Kit