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Human PRG4 ELISA Kit

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  • Alternative name

    Human lubricin ELISA Kit;Human Megakaryocyte-stimulating factor ELISA Kit;Human superficial zone proteoglycan ELISA Kit;Human MSF ELISA Kit;Human SZP ELISA Kit;Human CACP ELISA Kit;Human HAPO ELISA Kit;Human JCAP ELISA Kit;Human proteoglycan 4 ELISA Kit;Human articular superficial zone protein ELISA Kit;Human hemangiopoietin ELISA Kit;Human megakaryocyte stimulating factor ELISA Kit;
  • Catalog
    E032043
  • species
    Human
  • GenePRG4
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Proteoglycan 4 (PRG4). No significant cross-reactivity or interference between Proteoglycan 4 (PRG4) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 1.49ng/mL.
  • Intended UseHuman PRG4 ELISA Kit allows for the in vitro quantitative determination of PRG4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteoglycan 4 (PRG4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteoglycan 4 (PRG4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteoglycan 4 (PRG4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proteoglycan 4 (PRG4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Proteoglycan 4 C-terminal part Protein information
  • Uniprot ID PRG4_HUMAN
  • Uniprot AC Q92954; Q6DNC4; Q6DNC5; Q6ZMZ5; Q9BX49;
  • UniGene Hs.647723;
  • GeneID 10216
  • KEGG hsa:10216;
  • Human Proteoglycan 4 C-terminal part Protein SEQUENCE

  • SEQUENCE 1404 AA; 151077 MW; 782A11746B3FDEE5 CRC64;

    MAWKTLPIYL LLLLSVFVIQ QVSSQDLSSC AGRCGEGYSR DATCNCDYNC

    QHYMECCPDF KRVCTAELSC KGRCFESFER GRECDCDAQC KKYDKCCPDY

    ESFCAEVHNP TSPPSSKKAP PPSGASQTIK STTKRSPKPP NKKKTKKVIE

    SEEITEEHSV SENQESSSSS SSSSSSSTIR KIKSSKNSAA NRELQKKLKV

    KDNKKNRTKK KPTPKPPVVD EAGSGLDNGD FKVTTPDTST TQHNKVSTSP

    KITTAKPINP RPSLPPNSDT SKETSLTVNK ETTVETKETT TTNKQTSTDG

    KEKTTSAKET QSIEKTSAKD LAPTSKVLAK PTPKAETTTK GPALTTPKEP

    TPTTPKEPAS TTPKEPTPTT IKSAPTTPKE PAPTTTKSAP TTPKEPAPTT

    TKEPAPTTPK EPAPTTTKEP APTTTKSAPT TPKEPAPTTP KKPAPTTPKE

    PAPTTPKEPT PTTPKEPAPT TKEPAPTTPK EPAPTAPKKP APTTPKEPAP

    TTPKEPAPTT TKEPSPTTPK EPAPTTTKSA PTTTKEPAPT TTKSAPTTPK

    EPSPTTTKEP APTTPKEPAP TTPKKPAPTT PKEPAPTTPK EPAPTTTKKP

    APTTPKEPAP TTPKETAPTT PKKLTPTTPE KLAPTTPEKP APTTPEELAP

    TTPEEPTPTT PEEPAPTTPK AAAPNTPKEP APTTPKEPAP TTPKEPAPTT

    PKETAPTTPK GTAPTTLKEP APTTPKKPAP KELAPTTTKE PTSTTCDKPA

    PTTPKGTAPT TPKEPAPTTP KEPAPTTPKG TAPTTLKEPA PTTPKKPAPK

    ELAPTTTKGP TSTTSDKPAP TTPKETAPTT PKEPAPTTPK KPAPTTPETP

    PPTTSEVSTP TTTKEPTTIH KSPDESTPEL SAEPTPKALE NSPKEPGVPT

    TKTPAATKPE MTTTAKDKTT ERDLRTTPET TTAAPKMTKE TATTTEKTTE

    SKITATTTQV TSTTTQDTTP FKITTLKTTT LAPKVTTTKK TITTTEIMNK

    PEETAKPKDR ATNSKATTPK PQKPTKAPKK PTSTKKPKTM PRVRKPKTTP

    TPRKMTSTMP ELNPTSRIAE AMLQTTTRPN QTPNSKLVEV NPKSEDAGGA

    EGETPHMLLR PHVFMPEVTP DMDYLPRVPN QGIIINPMLS DETNICNGKP

    VDGLTTLRNG TLVAFRGHYF WMLSPFSPPS PARRITEVWG IPSPIDTVFT

    RCNCEGKTFF FKDSQYWRFT NDIKDAGYPK PIFKGFGGLT GQIVAALSTA

    KYKNWPESVY FFKRGGSIQQ YIYKQEPVQK CPGRRPALNY PVYGETTQVR

    RRRFERAIGP SQTHTIRIQY SPARLAYQDK GVLHNEVKVS ILWRGLPNVV

    TSAISLPNIR KPDGYDYYAF SKDQYYNIDV PSRTARAITT RSGQTLSKVW

    YNCP

  • UCSC uc001grt.5; human. [Q92954-1];



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