Human differentiation-dependent protein A4 ELISA Kit;Human intestinal membrane A4 protein ELISA Kit;Human A4 ELISA Kit;Human A4LSB ELISA Kit;Human proteolipid protein 2 ELISA Kit;Human A4 differentiation-dependent protein ELISA Kit;Human proteolipid protein 2 (colonic epithelium-enriched) ELISA Kit;
Catalog
E032055
species
Human
GenePLP2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2).
No significant cross-reactivity or interference between Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2) and analogues was observed.
Intended UseHuman PLP2 ELISA Kit allows for the in vitro quantitative determination of PLP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.